Ran Tao, Li Hengzhi, Liu Yong, Zhou Chuanshe, Tang Shaoxun, Han Xuefeng, Wang Min, He Zhixiong, Kang Jinghe, Yan Qiongxian, Tan Zhiliang, Beauchemin Karen A
Key Laboratory for Agro-Ecological Processes in Subtropical Region and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences , Changsha, Hunan 410125, People's Republic of China.
Graduate University of the Chinese Academy of Sciences , Beijing 100049, People's Republic of China.
J Agric Food Chem. 2016 Mar 23;64(11):2333-41. doi: 10.1021/acs.jafc.5b06131. Epub 2016 Mar 8.
G-protein-coupled receptor 120 (GPR120) is reported as a long-chain fatty acid (LCFA) receptor that elicits free fatty acid (FFA) regulation on metabolism homeostasis. The study aimed to clone the gpr120 gene of goats (g-GPR120) and subsequently investigate phylogenetic analysis and tissue distribution throughout the digestive tracts of kid goats, as well as the effect of housing versus grazing (H vs G) feeding systems on GPR120 expression. Partial coding sequence (CDS) of g-GPR120 was cloned and submitted to NCBI (accession no. KU161270 ). Phylogenetic analysis revealed that g-GPR120 shared higher homology in both mRNA and amino acid sequences for ruminants than nonruminants. Immunochemistry, real-time PCR, and Western blot analysis showed that g-GPR120 was expressed throughout the digestive tracts of goats. The expression of g-GPR120 was affected by feeding system and age, with greater expression of g-GPR120 in the G group. It was concluded that the g-GPR120-mediated LCFA chemosensing mechanism is widely present in the tongue and gastrointestinal tract of goats and that its expression can be affected by feeding system and age.
据报道，G蛋白偶联受体120（GPR120）是一种长链脂肪酸（LCFA）受体，可引发游离脂肪酸（FFA）对代谢稳态的调节。本研究旨在克隆山羊的gpr120基因（g-GPR120），随后对幼山羊消化道进行系统发育分析和组织分布研究，以及舍饲与放牧（H vs G）饲养系统对GPR120表达的影响。克隆了g-GPR120的部分编码序列（CDS）并提交至NCBI（登录号：KU161270）。系统发育分析表明，g-GPR120在反刍动物的mRNA和氨基酸序列上比非反刍动物具有更高的同源性。免疫化学、实时PCR和蛋白质印迹分析表明，g-GPR120在山羊的整个消化道中均有表达。g-GPR120的表达受饲养系统和年龄的影响，在G组中g-GPR120的表达更高。得出的结论是，g-GPR120介导的LCFA化学感应机制广泛存在于山羊的舌和胃肠道中，并且其表达会受到饲养系统和年龄的影响。
