Lee Sung-Eun, Wang Feng, Trujillo-Ocampo Abel, Ruiz-Vasquez Wilfredo, Cho Hyun-Woo, Takahashi Koichi, Molldrem Jeffrey J, Futreal Andrew, Garcia-Manero Guillermo, Im Jin S
Department of Stem Cell Transplantation and Cellular Therapy, Division of Cancer Medicine The University of Texas M.D. Anderson Cancer Center Houston Texas USA.
Department of Hematology, Seoul St. Mary's Hospital, College of Medicine The Catholic University of Korea Seoul South Korea.
EJHaem. 2020 Oct 5;1(2):552-557. doi: 10.1002/jha2.112. eCollection 2020 Nov.
The aim of this study is to investigate whether the peripheral blood (PB) can serve as a surrogate immune-microenvironment to bone marrow for genetic and immune monitoring in myelodysplastic syndrome (MDS). We compared the composition of T cell subsets and somatic mutation burden in 36 pairs of PB and matching bone marrow aspirate (BMA) using multi-parameter flow cytometry and NGS-based targeted sequencing analysis, respectively. Our immune-subset and NGS-based mutation analysis of BMA showed significant concordance with those of PB in MDS. Therefore, PB can provide easily accessible tumor immune-microenvironment for monitoring in the immune and genetic landscapes for MDS patients.
本研究的目的是调查外周血（PB）是否可作为骨髓的替代免疫微环境，用于骨髓增生异常综合征（MDS）的基因和免疫监测。我们分别使用多参数流式细胞术和基于NGS的靶向测序分析，比较了36对PB和匹配的骨髓穿刺液（BMA）中T细胞亚群的组成和体细胞突变负担。我们对BMA进行的免疫亚群和基于NGS的突变分析显示，与MDS患者PB的分析结果具有显著一致性。因此，PB可为MDS患者的免疫和基因图谱监测提供易于获取的肿瘤免疫微环境。
