Guo Xin, Huang Zhijie, Chen Jin, Hu Jiarui, Hu Die, Peng Daoquan, Yu Bilian
Department of Cardiovascular Medicine, Research Institute of Blood Lipid and Atherosclerosis, The Second Xiangya Hospital, Central South University, Changsha, China.
Department of Spine Surgery, The Second Xiangya Hospital, Central South University, Changsha, China.
Front Cardiovasc Med. 2022 Jun 29;9:913363. doi: 10.3389/fcvm.2022.913363. eCollection 2022.
It is well-established that there exists an inverse relationship between high-density lipoprotein (HDL) cholesterol and triglyceride (TG) levels in the plasma. However, information is lacking on the impact of post-prandial triglyceride-rich lipoproteins (TRLs) on the structure of HDL subclasses in patients with coronary artery disease (CAD). In this study, the data of 49 patients with CAD were analyzed to evaluate dynamic alterations in post-prandial lipid profiles using nuclear magnetic resonance-based methods. An enzyme-linked immunosorbent assay was used to quantify the serum angiopoietin-like protein 3 (ANGPTL3). After glucose supplementation, the expression of hepatic ANGPTL3 was evaluated both and . Compared to fasting levels, the post-prandial serum TG level of all participants was considerably increased. Although post-prandial total cholesterol in HDL (HDL-C) remained unchanged, free cholesterol in HDL particles (HDL-FC) was significantly reduced after a meal. Furthermore, the post-prandial decrease in the HDL-FC level corresponded to the increase in remnant cholesterol (RC), indicating the possible exchange of free cholesterol between HDL and TRLs after a meal. Moreover, CAD patients with exaggerated TG response to diet, defined as TG increase >30%, tend to have a greater post-prandial increase of RC and decrease of HDL-FC compared to those with TG increase ≤30%. Mechanistically, the fasting and post-prandial serum ANGPTL3 levels were significantly lower in those with TG increase ≤30% than those with TG increase >30%, suggesting that ANGPTL3, the key lipolysis regulator, may be responsible for the different post-prandial responses of TG, RC, and HDL-FC.
众所周知，血浆中高密度脂蛋白（HDL）胆固醇与甘油三酯（TG）水平呈负相关。然而，关于餐后富含甘油三酯的脂蛋白（TRL）对冠心病（CAD）患者HDL亚类结构的影响，目前仍缺乏相关信息。在本研究中，分析了49例CAD患者的数据，采用基于核磁共振的方法评估餐后血脂谱的动态变化。采用酶联免疫吸附测定法对血清血管生成素样蛋白3（ANGPTL3）进行定量。在补充葡萄糖后，分别在[此处原文缺失时间点信息]评估肝脏ANGPTL3的表达。与空腹水平相比，所有参与者的餐后血清TG水平均显著升高。尽管餐后HDL中的总胆固醇（HDL-C）保持不变，但餐后HDL颗粒中的游离胆固醇（HDL-FC）显著降低。此外，餐后HDL-FC水平的降低与残余胆固醇（RC）的增加相对应，表明餐后HDL与TRL之间可能存在游离胆固醇的交换。此外，与TG升高≤30%的患者相比，对饮食中TG反应过度（定义为TG升高>30%）的CAD患者，餐后RC升高和HDL-FC降低的幅度往往更大。从机制上讲，TG升高≤30%的患者空腹和餐后血清ANGPTL3水平显著低于TG升高>30%的患者，这表明关键的脂解调节因子ANGPTL3可能是导致TG、RC和HDL-FC餐后不同反应的原因。
