Groener J E, Scheek L M, van Ramshorst E, Krauss X H, van Tol A
Department of Biochemistry, Cardiovascular Research Institute (COEUR), Erasmus University, Rotterdam, The Netherlands.
Atherosclerosis. 1998 Apr;137(2):311-9. doi: 10.1016/s0021-9150(97)00287-6.
We studied the effect of a single oral fat load, supplemented with retinyl palmitate (RP), on high density lipoprotein (HDL) lipids in six normolipidemic men with coronary artery disease (CAD) and in six age- and lipid-matched controls. All subjects were selected from a study group which underwent the same protocol 2 years earlier. Post-prandial total plasma lipids, plasma RP levels, and HDL lipids were evaluated at 2-h intervals up till 10 h after the meal. In most subjects the post-prandial response of plasma triglyceride (TG) and plasma RP was identical in the first and second tests. Following the fat load, control subjects showed no change in HDL total cholesterol (TC) or HDL cholesteryl ester (CE) and showed an increase in HDL-TG. CAD subjects however showed a decrease in HDL-TC and HDL-CE and an increase in HDL-TG, similar to the increase in control subjects. In control subjects an increase in HDL phospholipid (PL) was apparent between 0 and 8 h after the fat load. By contrast, in CAD subjects the increase in HDL-PL was only found after as long as 6 h. The magnitude of the post-prandial response of HDL-PL measured during the test was significantly lower in the CAD group. The effects of the fat load on HDL free cholesterol (FC) were similar to the changes in HDL-PL. These data support the hypothesis that PL and FC released during the degradation of chylomicrons as surface remnants are taken up by HDL. This process is clearly delayed in normolipidemic CAD subjects compared with controls. The data suggest that differences in the post-prandial response to an oral fat load in normolipidemic CAD patients and control subjects are not confined to the clearance of TG-rich lipoproteins, but also involve a difference in the uptake of chylomicron surface material by HDL.
我们研究了单次口服脂肪负荷（补充棕榈酸视黄酯，RP）对6名患有冠状动脉疾病（CAD）的血脂正常男性以及6名年龄和血脂匹配的对照者高密度脂蛋白（HDL）脂质的影响。所有受试者均选自2年前接受相同方案的研究组。在进食后直至10小时期间，每隔2小时评估餐后总血浆脂质、血浆RP水平和HDL脂质。在大多数受试者中，第一次和第二次测试时血浆甘油三酯（TG）和血浆RP的餐后反应相同。脂肪负荷后，对照者的HDL总胆固醇（TC）或HDL胆固醇酯（CE）无变化，HDL-TG增加。然而，CAD患者的HDL-TC和HDL-CE降低，HDL-TG增加，与对照者的增加情况相似。在对照者中，脂肪负荷后0至8小时HDL磷脂（PL）明显增加。相比之下，CAD患者中HDL-PL的增加仅在长达6小时后才出现。测试期间测量的HDL-PL餐后反应幅度在CAD组中显著较低。脂肪负荷对HDL游离胆固醇（FC）的影响与HDL-PL的变化相似。这些数据支持以下假设：乳糜微粒降解过程中作为表面残余物释放的PL和FC被HDL摄取。与对照者相比，血脂正常的CAD患者中这一过程明显延迟。数据表明，血脂正常的CAD患者和对照者对口服脂肪负荷的餐后反应差异不仅限于富含TG脂蛋白的清除，还涉及HDL对乳糜微粒表面物质摄取的差异。
