• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

构建结肠癌中与外泌体相关的 miRNA-mRNA 网络。

Construction of a miRNA-mRNA Network Related to Exosomes in Colon Cancer.

机构信息

Department of Medical Oncology, Lu'an Hospital Affiliated to Anhui University of Chinese Medicine, China.

出版信息

Dis Markers. 2022 Jul 5;2022:2192001. doi: 10.1155/2022/2192001. eCollection 2022.

DOI:10.1155/2022/2192001
PMID:35845138
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9277152/
Abstract

BACKGROUND

The competing endogenous RNA (CeRNA) network plays important roles in the occurrence and development of colon cancer. This research is aimed at constructing a miRNA-mRNA network associated with exosomes in colon cancer.

METHODS

We explored the GEO database and then analyzed the RNAs of 722 samples to obtain differentially expressed miRNAs (DEMs) and mRNAs (DEGs) alongside the progress of colon cancer. Next, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of DEM target genes and DEGs were performed. In addition, a miRNA-mRNA network related to exosomes in colon cancer was constructed based on DEMs and DEGs. Finally, the expression of miRNA and mRNA in the network was verified by GEPIA2 on the base of TCGA database.

RESULTS

Through our analysis, 19 DEMs (17 up and 2 down) and 1672 DEGs (954 up and 718 down) were screened. The GO and KEGG results show that these DEGs were mainly enriched in ribonucleoprotein complex biogenesis, noncoding RNA metabolic process, cell-substrate junction, cadherin binding, transcription coregulator activity, and regulation of the human T-cell leukemia virus 1 infection-related pathway. Besides, a miRNA-mRNA network, including 4 miRNAs (hsa-miR-623, hsa-miR-320c, hsa-miR-486-5p, and hsa-miR-1290) and 7 mRNAs (GNAI1, CADM1, PGRMC2, etc.), was constructed. Three of these seven mRNAs were downregulated in colon cancer. Ultimately, the GNAI1, CADM1, and PGRMC2 expression levels were verified by TCGA database.

CONCLUSIONS

This study reveals the network relationship between colon cancer exosome-derived miRNA and targeted mRNA. It deepens our understanding of new molecular mechanisms and pathways that may play a role in the occurrence and metastasis of colon cancer.

摘要

背景

竞争内源性 RNA（CeRNA）网络在结肠癌的发生和发展中发挥重要作用。本研究旨在构建与结肠癌外泌体相关的 miRNA-mRNA 网络。

方法

我们探索了 GEO 数据库，然后分析了 722 个样本的 RNA，以获得结肠癌进展过程中差异表达的 miRNAs（DEMs）和 mRNAs（DEGs）。接下来，对 DEM 靶基因和 DEGs 进行基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析。此外，基于 DEMs 和 DEGs 构建了与结肠癌外泌体相关的 miRNA-mRNA 网络。最后，基于 TCGA 数据库，通过 GEPIA2 验证网络中 miRNA 和 mRNA 的表达。

结果

通过分析，筛选出 19 个 DEM（17 个上调和 2 个下调）和 1672 个 DEGs（954 个上调和 718 个下调）。GO 和 KEGG 结果表明，这些 DEGs 主要富集在核糖核蛋白复合物生物发生、非编码 RNA 代谢过程、细胞-基质连接、钙黏蛋白结合、转录共激活因子活性以及调节人类 T 细胞白血病病毒 1 感染相关途径。此外，构建了一个包括 4 个 miRNA（hsa-miR-623、hsa-miR-320c、hsa-miR-486-5p 和 hsa-miR-1290）和 7 个 mRNAs（GNAI1、CADM1、PGRMC2 等）的 miRNA-mRNA 网络。这 7 个 mRNAs 中有 3 个在结肠癌中下调。最终，通过 TCGA 数据库验证了 GNAI1、CADM1 和 PGRMC2 的表达水平。

结论

本研究揭示了结肠癌外泌体衍生 miRNA 与靶向 mRNA 之间的网络关系，加深了我们对可能在结肠癌发生和转移中发挥作用的新分子机制和途径的理解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7377/9277152/316f285e9b77/DM2022-2192001.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7377/9277152/bf95e84233ea/DM2022-2192001.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7377/9277152/753f4e0d9022/DM2022-2192001.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7377/9277152/7c517321afd5/DM2022-2192001.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7377/9277152/60695cae0fc3/DM2022-2192001.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7377/9277152/acef91b060e2/DM2022-2192001.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7377/9277152/7a7f8a290020/DM2022-2192001.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7377/9277152/ca7bf78ef5b2/DM2022-2192001.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7377/9277152/316f285e9b77/DM2022-2192001.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7377/9277152/bf95e84233ea/DM2022-2192001.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7377/9277152/753f4e0d9022/DM2022-2192001.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7377/9277152/7c517321afd5/DM2022-2192001.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7377/9277152/60695cae0fc3/DM2022-2192001.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7377/9277152/acef91b060e2/DM2022-2192001.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7377/9277152/7a7f8a290020/DM2022-2192001.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7377/9277152/ca7bf78ef5b2/DM2022-2192001.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7377/9277152/316f285e9b77/DM2022-2192001.008.jpg

相似文献

1
Construction of a miRNA-mRNA Network Related to Exosomes in Colon Cancer.构建结肠癌中与外泌体相关的 miRNA-mRNA 网络。
Dis Markers. 2022 Jul 5;2022:2192001. doi: 10.1155/2022/2192001. eCollection 2022.
2
Construction of a Competitive Endogenous RNA Network Related to Exosomes in Diabetic Retinopathy.构建与糖尿病视网膜病变相关外泌体的竞争性内源性 RNA 网络。
Comb Chem High Throughput Screen. 2023;26(3):576-588. doi: 10.2174/1386207325666220610122114.
3
Construction of an exosome-associated miRNA-mRNA regulatory network and validation of and miR-17-5p as potential biomarkers associated with ovarian cancer.构建外泌体相关的miRNA-mRNA调控网络并验证miR-17-5p作为与卵巢癌相关的潜在生物标志物。
Transl Cancer Res. 2024 Feb 29;13(2):1052-1067. doi: 10.21037/tcr-23-940. Epub 2024 Feb 28.
4
Construction of Endometrial Carcinoma ceRNA Network and Screening of Key Genes Based on TCGA Database.基于 TCGA 数据库构建子宫内膜癌 ceRNA 网络及关键基因的筛选。
Comput Math Methods Med. 2022 Jul 4;2022:1418232. doi: 10.1155/2022/1418232. eCollection 2022.
5
LINC01018 and SMIM25 sponged miR-182-5p in endometriosis revealed by the ceRNA network construction.LINC01018 和 SMIM25 通过 ceRNA 网络构建揭示了子宫内膜异位症中的 miR-182-5p 海绵作用。
Int J Immunopathol Pharmacol. 2020 Jan-Dec;34:2058738420976309. doi: 10.1177/2058738420976309.
6
Construction of the miRNA-mRNA regulatory network and analysis of hub genes in oral squamous cell carcinoma.构建 miRNA-mRNA 调控网络并分析口腔鳞状细胞癌中的枢纽基因。
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2022 Sep;166(3):280-289. doi: 10.5507/bp.2022.001. Epub 2022 Feb 3.
7
Identification of cell cycle as the critical pathway modulated by exosome-derived microRNAs in gallbladder carcinoma.鉴定细胞周期为外泌体衍生 microRNAs 调节的胆囊癌关键通路。
Med Oncol. 2021 Oct 16;38(12):141. doi: 10.1007/s12032-021-01594-8.
8
Bioinformatic analysis reveals an exosomal miRNA-mRNA network in colorectal cancer.生物信息学分析揭示结直肠癌中外泌体 miRNA-mRNA 网络。
BMC Med Genomics. 2021 Feb 27;14(1):60. doi: 10.1186/s12920-021-00905-2.
9
Predictions of the dysregulated competing endogenous RNA signature involved in the progression of human lung adenocarcinoma.预测涉及人肺腺癌进展的失调竞争内源性 RNA 特征。
Cancer Biomark. 2020;29(3):399-416. doi: 10.3233/CBM-200133.
10
A Competing Endogenous RNA Network Reveals Novel Potential lncRNA, miRNA, and mRNA Biomarkers in the Prognosis of Human Colon Adenocarcinoma.竞争性内源性 RNA 网络揭示了人类结肠腺癌预后的新型潜在 lncRNA、miRNA 和 mRNA 生物标志物。
J Surg Res. 2019 Mar;235:22-33. doi: 10.1016/j.jss.2018.09.053. Epub 2018 Oct 23.

引用本文的文献

1
Exosomal long noncoding RNAs and microRNAs in colorectal cancer.结直肠癌中的外泌体长链非编码RNA和微小RNA
Tzu Chi Med J. 2025 Jun 30;37(3):235-246. doi: 10.4103/tcmj.tcmj_62_25. eCollection 2025 Jul-Sep.
2
Integrating plasma exosomal miRNAs, ultrasound radiomics and tPSA for the diagnosis and prediction of early prostate cancer: a multi-center study.整合血浆外泌体微小RNA、超声影像学和总前列腺特异性抗原用于早期前列腺癌的诊断和预测：一项多中心研究
Clin Transl Oncol. 2025 Mar;27(3):1248-1262. doi: 10.1007/s12094-024-03682-3. Epub 2024 Aug 28.
3
Analysis of mRNA-miRNA interaction network reveals the role of CAFs-derived exosomes in the immune regulation of oral squamous cell carcinoma.

本文引用的文献

1
clusterProfiler 4.0: A universal enrichment tool for interpreting omics data.clusterProfiler 4.0：用于解释组学数据的通用富集工具。
Innovation (Camb). 2021 Jul 1;2(3):100141. doi: 10.1016/j.xinn.2021.100141. eCollection 2021 Aug 28.
2
Identifying causal models between genetically regulated methylation patterns and gene expression in healthy colon tissue.鉴定健康结肠组织中基因调控的甲基化模式与基因表达之间的因果模型。
Clin Epigenetics. 2021 Aug 21;13(1):162. doi: 10.1186/s13148-021-01148-9.
3
Exosomal miR-183-5p Shuttled by M2 Polarized Tumor-Associated Macrophage Promotes the Development of Colon Cancer Targeting THEM4 Mediated PI3K/AKT and NF-κB Pathways.
分析 mRNA-miRNA 相互作用网络揭示了 CAFs 衍生的外泌体在口腔鳞状细胞癌免疫调控中的作用。
BMC Cancer. 2023 Jun 26;23(1):591. doi: 10.1186/s12885-023-11028-5.
由M2极化的肿瘤相关巨噬细胞转运的外泌体miR-183-5p通过靶向THEM4介导的PI3K/AKT和NF-κB通路促进结肠癌的发展。
Front Oncol. 2021 Jun 25;11:672684. doi: 10.3389/fonc.2021.672684. eCollection 2021.
4
HOXA13 in etiology and oncogenic potential of Barrett's esophagus.HOXA13 在巴雷特食管的病因学和致癌潜能中的作用。
Nat Commun. 2021 Jun 7;12(1):3354. doi: 10.1038/s41467-021-23641-8.
5
Hsa-miR-125b Therapeutic Role in Colon Cancer Is Dependent on the Mutation Status of the TP53 Gene.Hsa-miR-125b在结肠癌中的治疗作用取决于TP53基因的突变状态。
Pharmaceutics. 2021 May 6;13(5):664. doi: 10.3390/pharmaceutics13050664.
6
Exosomes and extracellular vesicles: Rethinking the essential values in cancer biology.外泌体和细胞外囊泡：重新思考癌症生物学中的基本价值。
Semin Cancer Biol. 2021 Sep;74:79-91. doi: 10.1016/j.semcancer.2021.03.032. Epub 2021 Mar 31.
7
M2 macrophage-derived exosomal microRNA-155-5p promotes the immune escape of colon cancer by downregulating ZC3H12B.M2巨噬细胞衍生的外泌体微小RNA-155-5p通过下调ZC3H12B促进结肠癌的免疫逃逸。
Mol Ther Oncolytics. 2021 Feb 6;20:484-498. doi: 10.1016/j.omto.2021.02.005. eCollection 2021 Mar 26.
8
Exosomes and Cell Communication: From Tumour-Derived Exosomes and Their Role in Tumour Progression to the Use of Exosomal Cargo for Cancer Treatment.外泌体与细胞通讯：从肿瘤来源的外泌体及其在肿瘤进展中的作用到利用外泌体所载物质进行癌症治疗
Cancers (Basel). 2021 Feb 16;13(4):822. doi: 10.3390/cancers13040822.
9
PGRMC Proteins Are Coming of Age: A Special Issue on the Role of PGRMC1 and PGRMC2 in Metabolism and Cancer Biology.PGRMC蛋白渐趋成熟：关于PGRMC1和PGRMC2在代谢与癌症生物学中作用的特刊
Cancers (Basel). 2021 Jan 29;13(3):512. doi: 10.3390/cancers13030512.
10
IRF4 overexpression promotes the transdifferentiation of tregs into macrophage-like cells to inhibit the development of colon cancer.IRF4过表达促进调节性T细胞向巨噬细胞样细胞转分化，从而抑制结肠癌的发展。
Cancer Cell Int. 2021 Jan 19;21(1):58. doi: 10.1186/s12935-021-01766-6.