Wu Yong-Fang, Fang Da-Lang, Wei Jie
Department of Burn and Plastic Surgery, the People's Hospital of Baise, Baise, China.
Department of Breast and Thyroid Surgery, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China.
Ann Transl Med. 2022 Jun;10(12):680. doi: 10.21037/atm-22-2033.
The skin is the most exposed tissue and has multiple functions. Wound healing is a major medical problem due to trauma and pathophysiological alterations suffered by patients. The aim of the present study was to search for potential autophagy genes associated with wound healing.
The GSE168760 dataset was obtained from the Gene Expression Omnibus (GEO) database, and sequencing results were obtained for 14 patient traumas at different time periods. Differentially expressed gene (DEG) analysis, Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were performed. Immune cell and correlation analysis were performed for autophagy genes and DEGs. Peripheral blood was collected from patients at different time periods and Western blot (WB) assay was performed to verify autophagy genes.
A total of 226 DEGs were screened on days 0, 7, and 14, of which 162 genes were upregulated and 64 genes were downregulated. Of these, eukaryotic translation initiation factor 2-alpha kinase 2 () and retinoblastoma 1 () were autophagy-associated genes. The DEGs were mainly involved in response to virus, cellular response to type I interferon Epstein-Barr virus infection, human papillomavirus infection, ribosome, hepatitis B and RIG-I-like. and showed positive correlation with some of the immune cells, and WB showed that and proteins were significantly increased with wound healing.
The comprehensive analysis of GEO data in the present study provides a new theoretical basis for the molecular pathogenesis of trauma healing and potential autophagy-related therapeutic targets.
皮肤是最易暴露的组织，具有多种功能。由于患者遭受创伤和病理生理改变，伤口愈合是一个主要的医学问题。本研究的目的是寻找与伤口愈合相关的潜在自噬基因。
从基因表达综合数据库（GEO）中获取GSE168760数据集，并获得了14例患者在不同时间段的创伤测序结果。进行了差异表达基因（DEG）分析、基因本体（GO）和京都基因与基因组百科全书（KEGG）富集分析。对自噬基因和DEG进行了免疫细胞和相关性分析。在不同时间段采集患者外周血，并进行蛋白质免疫印迹（WB）检测以验证自噬基因。
在第0天、第7天和第14天共筛选出226个DEG，其中162个基因上调，64个基因下调。其中，真核翻译起始因子2α激酶2（）和成视网膜细胞瘤蛋白1（）是自噬相关基因。这些DEG主要参与对病毒的反应、细胞对I型干扰素的反应、EB病毒感染、人乳头瘤病毒感染、核糖体、乙型肝炎和视黄酸诱导基因I样反应。 和 与一些免疫细胞呈正相关，WB显示 和 蛋白随伤口愈合显著增加。
本研究对GEO数据的综合分析为创伤愈合的分子发病机制和潜在的自噬相关治疗靶点提供了新的理论依据。
