胰岛素样生长因子 2 mRNA 结合蛋白 3 及其相关分子作为小细胞肺癌潜在的生物标志物。
Insulin-Like Growth Factor 2 mRNA-Binding Protein 3 and Its Related Molecules as Potential Biomarkers in Small-Cell Lung Cancer.
机构信息
Department of Respiratory, Kunshan First People's Hospital, Suzhou 215004, China.
Department of Oncology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
出版信息
Biomed Res Int. 2022 Jul 7;2022:5774339. doi: 10.1155/2022/5774339. eCollection 2022.
BACKGROUND
Insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) plays a key role in tumorigenesis and tumor progression. Lung cancer is the leading cause of cancer-related death in men and women all over the world. However, the relationship between IGF2BP3 and small-cell lung cancer (SCLC) has not been reported yet.
METHODS
SCLC and normal samples (GSE19945 and GSE149507) were obtained in the Gene Expression Omnibus (GEO) dataset. Differential genes were screened by R software, and functional analysis and signal pathway enrichment analysis were carried out. In addition, we used the survival analysis database to analyze the relationship between prognosis and gene expression. Besides, immunohistochemistry (IHC) and quantitative real-time PCR (qPCR) were used for further research.
RESULTS
Five differentially expressed miRNAs and 9 differentially expressed mRNAs were selected by using R software. Survival analysis database results show that C7, CLIC5, PRDX1, IGF2BP3, and LDB2 were related the overall survival of patients with SCLC. Furthermore, multivariate analysis included that IGF2BP3 was independent risk factors for SCLC patients. Besides, gene function and signal pathway enrichment analysis showed that differentially expressed miRNAs were involved in the process of tumorigenesis and development. Furthermore, IHC and qPCR outcomes showed that the expression level of hsa-miR-182, hsa-miR-183, and IGF2BP3 was differentially expressed in normal lung tissues (NLTs) and SCLC tissues (SCLCTs).
CONCLUSIONS
Our results concluded that hsa-miR-182, hsa-miR-183, and IGF2BP3 may take part in the development of SCLC.
背景
胰岛素样生长因子 2mRNA 结合蛋白 3（IGF2BP3）在肿瘤发生和肿瘤进展中起关键作用。肺癌是全世界男性和女性癌症相关死亡的主要原因。然而，IGF2BP3 与小细胞肺癌（SCLC）之间的关系尚未报道。
方法
从基因表达综合数据库（GEO）中获取 SCLC 和正常样本（GSE19945 和 GSE149507）。使用 R 软件筛选差异基因，并进行功能分析和信号通路富集分析。此外，我们使用生存分析数据库分析了预后与基因表达的关系。此外，还进行了免疫组织化学（IHC）和定量实时 PCR（qPCR）进一步研究。
结果
使用 R 软件筛选出 5 个差异表达的 miRNA 和 9 个差异表达的 mRNA。生存分析数据库结果表明，C7、CLIC5、PRDX1、IGF2BP3 和 LDB2 与 SCLC 患者的总生存期相关。此外，多因素分析包括 IGF2BP3 是 SCLC 患者的独立危险因素。此外，基因功能和信号通路富集分析表明，差异表达的 miRNA 参与了肿瘤发生和发展的过程。此外，IHC 和 qPCR 结果表明，hsa-miR-182、hsa-miR-183 和 IGF2BP3 在正常肺组织（NLTs）和 SCLC 组织（SCLCTs）中的表达水平存在差异。
结论
我们的研究结果表明 hsa-miR-182、hsa-miR-183 和 IGF2BP3 可能参与 SCLC 的发生。
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