Davis A P, Foote R H
J Dairy Sci. 1987 Apr;70(4):850-7. doi: 10.3168/jds.S0022-0302(87)80083-8.
The relationship between bull non-return rate and percent acrosome-reacted and percent motile sperm treated with 24.8, 34.8, and 44.8 microM dilauroylphosphatidylcholine liposomes was examined. Frozen-thawed spermatozoa from six bulls, with fertility ranging from 64 to 78%, were incubated at 39 degrees C with the liposomes for 0, 7, and 15 min. Bulls differed in percentages of motile sperm, and this decreased over time, especially at the highest concentration of lipids (44.8 microM). Bulls differed initially in their percentage of sperm with intact acrosomes and in their linear rate of increase in percent acrosome-reacted sperm. At 24.8 microM of lipid the rate of increase was nearly linear, whereas at higher concentrations the maximum acrosome reaction was approached during short incubations. The variables 1) decline in percent motile sperm and 2) increase in percent acrosome-reacted sperm, used singly, predicted fertility most accurately when measured on sperm treated with 44.8 and 24.8 microM of lipids, respectively. However, the highest relationship (R2 greater than or equal to .97) was established by combining the acrosome and motility measurements of sperm exposed to 34.8 microM of lipids. Thus, potential fertility of bulls may be predicted by application of this assay to frozen-thawed semen.
研究了用24.8、34.8和44.8微摩尔二月桂酰磷脂酰胆碱脂质体处理的公牛不返情率与顶体反应精子百分比和活动精子百分比之间的关系。从6头生育力在64%至78%之间的公牛采集冻融精子，在39℃下与脂质体孵育0、7和15分钟。公牛的活动精子百分比存在差异，且随时间下降，尤其是在最高脂质浓度（44.8微摩尔）下。公牛最初在完整顶体精子百分比以及顶体反应精子百分比的线性增加速率方面存在差异。在24.8微摩尔脂质时，增加速率近乎呈线性，而在较高浓度下，短时间孵育期间接近最大顶体反应。变量1）活动精子百分比下降和2）顶体反应精子百分比增加，分别在经44.8和24.8微摩尔脂质处理的精子上测量时，单独使用能最准确地预测生育力。然而，通过结合暴露于34.8微摩尔脂质的精子的顶体和活力测量结果，建立了最高的相关性（R2大于或等于0.97）。因此，通过将该检测方法应用于冻融精液，可预测公牛的潜在生育力。
