Lenz R W, Martin J L, Bellin M E, Ax R L
Sire Power, Inc., Tunkhannock, PA 18657.
J Dairy Sci. 1988 Apr;71(4):1073-7. doi: 10.3168/jds.S0022-0302(88)79654-X.
Bull spermatozoa were incubated with 0 or 50 micrograms/ml chondroitin sulfates to induce acrosome reactions in vitro. Data were computed as percent increase in acrosome reactions elicited by chondroitin sulfates compared with control samples for each replicate. Data from 8 bulls with known nonreturn rates served as basis to predict nonreturn rates of 18 bulls entering an AI lineup. The average assay CV was 16.1% for all 26 bulls. Correlation coefficients of polynomial regression lines computed between actual nonreturn rates and percent increases in acrosome reactions in response to chondroitin sulfates were .98 and .81 for the 8 reference bulls and combined data of all 26 bulls, respectively. It is concluded that a laboratory assay using chondroitin sulfates to induce acrosome reactions in sperm can be used to predict relative fertility of bulls.
将公牛精子与 0 或 50 微克/毫升的硫酸软骨素一起孵育，以在体外诱导顶体反应。数据计算为与每个重复样本的对照样品相比，硫酸软骨素引发的顶体反应增加的百分比。来自 8 头已知不返情率的公牛的数据用作预测进入人工授精队列的 18 头公牛不返情率的基础。所有 26 头公牛的平均检测变异系数为 16.1%。对于 8 头参考公牛以及所有 26 头公牛的合并数据，实际不返情率与硫酸软骨素诱导的顶体反应增加百分比之间计算的多项式回归线的相关系数分别为 0.98 和 0.81。得出的结论是，使用硫酸软骨素诱导精子顶体反应的实验室检测可用于预测公牛的相对生育力。
