Zhu Ziying, Zhang Xiaona, Hao Haojie, Xu Heran, Shu Jun, Hou Qian, Wang Min
College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing, China.
The First Medical Center, Chinese People's Liberation Army General Hospital, Beijing, China.
Front Cell Neurosci. 2022 Jun 30;16:913009. doi: 10.3389/fncel.2022.913009. eCollection 2022.
Wound repair is a key step in the treatment of skin injury caused by burn, surgery, and trauma. Various stem cells have been proven to promote wound healing and skin regeneration as candidate seed cells. Therefore, exosomes derived from stem cells are emerging as a promising method for wound repair. However, the mechanism by which exosomes promote wound repair is still unclear. In this study, we reported that exosomes derived from umbilical cord mesenchymal stem cells (UC-MSCs) promote wound healing and skin regeneration by treating cutaneous nerve damage. The results revealed that UC-MSCs exosomes (UC-MSC-Exo) promote the growth and migration of dermal fibroblast cells. In culture, dermal fibroblasts could promote to nerve cells and secrete nerve growth factors when stimulated by exosomes. During the repair process UC-MSC-Exo accelerated the recruitment of fibroblasts at the site of trauma and significantly enhanced cutaneous nerve regeneration . Interestingly, it was found that UC-MSC-Exo could promote wound healing and skin regeneration by recruiting fibroblasts, stimulating them to secrete nerve growth factors (NGFs) and promoting skin nerve regeneration. Therefore, we concluded that UC-MSC-Exo promote cutaneous nerve repair, which may play an important role in wound repair and skin regeneration.
伤口修复是治疗由烧伤、手术和创伤引起的皮肤损伤的关键步骤。各种干细胞已被证明作为候选种子细胞可促进伤口愈合和皮肤再生。因此，源自干细胞的外泌体正成为一种有前景的伤口修复方法。然而，外泌体促进伤口修复的机制仍不清楚。在本研究中，我们报道源自脐带间充质干细胞（UC-MSCs）的外泌体通过治疗皮肤神经损伤促进伤口愈合和皮肤再生。结果显示，UC-MSCs外泌体（UC-MSC-Exo）促进真皮成纤维细胞的生长和迁移。在培养中，真皮成纤维细胞在受到外泌体刺激时可向神经细胞分化并分泌神经生长因子。在修复过程中，UC-MSC-Exo加速了创伤部位成纤维细胞的募集，并显著增强了皮肤神经再生。有趣的是，发现UC-MSC-Exo可通过募集成纤维细胞、刺激它们分泌神经生长因子（NGFs）以及促进皮肤神经再生来促进伤口愈合和皮肤再生。因此，我们得出结论，UC-MSC-Exo促进皮肤神经修复，这可能在伤口修复和皮肤再生中起重要作用。
