Kim Seung-Hun, Lee Mingyun, Choi Kwang-Hwan, Jeong Jinsol, Lee Dong-Kyung, Oh Jong-Nam, Choe Gyung Cheol, Lee Chang-Kyu
Department of Agricultural Biotechnology, Animal Biotechnology Major, And Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, Republic of Korea.
Research and Development Center, Space F Corporation, Hwasung, Gyeonggi-do 18471, Republic of Korea.
Stem Cells Int. 2022 Jun 11;2022:6337532. doi: 10.1155/2022/6337532. eCollection 2022.
The present study examined the activity and function of the pig enhancer in the porcine early embryonic development stage and porcine authentic embryonic stem cells. OCT4 is known as a pluripotent regulator, and its upstream regulatory region-based dual-fluorescence protein reporter system controlled by distal and proximal enhancers is broadly used in studies examining the states and mechanism of pluripotency. We analyzed how this reporter system functions during early embryo development and in stem cells using a previously established porcine-specific reporter system. We demonstrated that the porcine distal enhancer and proximal enhancer were activated with different expression patterns simultaneously as the expression of pluripotent marker genes changed during the development of in vitro pathenotes and the establishment of porcine embryonic stem cells (ESCs). This work demonstrates the applicability of the porcine upstream region-derived dual-fluorescence reporter system, which may be applied to investigations of species-specific pluripotency in porcine-origin cells. These reporter systems may be useful tools for studies of porcine-specific pluripotency, early embryo development, and embryonic stem cells.
本研究检测了猪增强子在猪早期胚胎发育阶段和猪源真性胚胎干细胞中的活性及功能。OCT4作为一种多能性调控因子，基于其上游调控区域、由远端和近端增强子控制的双荧光蛋白报告系统，广泛应用于多能性状态及机制的研究。我们利用先前建立的猪特异性报告系统，分析了该报告系统在早期胚胎发育和干细胞中的作用机制。我们证明，随着体外孤雌胚胎发育及猪胚胎干细胞（ESC）建立过程中多能性标记基因表达的变化，猪远端增强子和近端增强子以不同的表达模式同时被激活。这项工作证明了源自猪上游区域的双荧光报告系统的适用性，该系统可应用于猪源细胞中物种特异性多能性的研究。这些报告系统可能是研究猪特异性多能性、早期胚胎发育及胚胎干细胞的有用工具。
