Himuro Takahiro, Tsukamoto Shota, Saito Yoji
National Institute of Technology (KOSEN), Kure College, 2-2-11 Agaminami, Kure, Hiroshima 737-8506, Japan.
Seikei University, 3-3-1 Kichijoji-Kitamachi, Musashino-shi, Tokyo 180-8633, Japan.
ACS Omega. 2022 Jun 27;7(27):23549-23554. doi: 10.1021/acsomega.2c02069. eCollection 2022 Jul 12.
In this study, we developed a sensing device that can detect deoxyribonuclease (DNase) based on the electrical properties of deoxyribonucleic acid (DNA). We estimated the equivalent circuit between the electrodes with immobilized DNA and investigated whether the characteristics of the electrodes change before and after the DNase reaction. This method detects DNase by simply evaluating the electrical properties of DNA without using a fluorescent reagent. Therefore, inexpensive and highly accurate measurements can be performed with simple operations. However, detection sensitivity must be increased for practical feasibility. Hence, we investigated whether DNA immobilization is restricted by changing the shape of the electrode to a triangle with sharp edges, which may improve the sensitivity of DNase. Additionally, we attempted to detect DNase from an extremely small amount of sample solution using a microchannel. The device was able to quantitatively analyze DNase I activity with a detection limit of 5.5 × 10 unit/μL. The results demonstrate the effectiveness of the proposed sensing device for various medical applications.
在本研究中，我们开发了一种基于脱氧核糖核酸（DNA）电学性质来检测脱氧核糖核酸酶（DNase）的传感装置。我们估算了固定有DNA的电极之间的等效电路，并研究了DNase反应前后电极特性是否发生变化。该方法通过简单评估DNA的电学性质来检测DNase，无需使用荧光试剂。因此，能够以简单的操作进行低成本且高精度的测量。然而，为了实际可行性，必须提高检测灵敏度。因此，我们研究了将电极形状改变为具有尖锐边缘的三角形是否会限制DNA固定，这可能会提高DNase的灵敏度。此外，我们尝试使用微通道从极少量的样品溶液中检测DNase。该装置能够定量分析DNase I活性，检测限为5.5×10单位/μL。结果证明了所提出的传感装置在各种医学应用中的有效性。
