Key Laboratory of Colloid and Interface Chemistry, Shandong University, Ministry of Education, Jinan, 250100, China.
Infectious Disease Prevention and Control Section, Shandong Center for Disease Control and Prevention, Jinan, 250014, China.
Talanta. 2024 Jan 1;266(Pt 1):124994. doi: 10.1016/j.talanta.2023.124994. Epub 2023 Jul 26.
Deoxyribonuclease I (DNase I) is a biomarker which has important applications in various biological processes. Thus, it is highly important to develop a user-friendly method for the detection of DNase I. Here, we present a paper-based distance sensor for the rapid detection of DNase I based on changes in the viscosity of DNA mucus. The viscosity of DNA mucus varies with different concentrations of DNase I, showing different water flow lengths on the pH test papers, this makes the quantification of DNase I possible. This method has a wide linear range (0.01-10 U/mL), excellent sensitivity, remarkable specificity and excellent reproducibility. The detection limit reaches 0.003 U/mL. Additionally, it can be well applied to detection of DNase I inhibitors, assay of DNase I in human serum and quality evaluation of nucleic acid scavengers. In general, this study offers a brief, convenient, label-free, and economical method to construct paper-based distance sensors using DNA mucus, which is very promising in the detection of DNase I in various applications.
脱氧核糖核酸酶 I（DNase I）是一种在各种生物过程中具有重要应用的生物标志物。因此，开发一种用于检测 DNase I 的用户友好型方法非常重要。在这里，我们提出了一种基于 DNA 粘液粘度变化的用于快速检测 DNase I 的基于纸质的距离传感器。不同浓度的 DNase I 会导致 DNA 粘液的粘度发生变化，在 pH 试纸上显示出不同的水流长度，这使得对 DNase I 的定量检测成为可能。该方法具有较宽的线性范围（0.01-10 U/mL）、优异的灵敏度、显著的特异性和良好的重现性。检测限达到 0.003 U/mL。此外，它可以很好地应用于 DNase I 抑制剂的检测、人血清中 DNase I 的测定以及核酸清除剂的质量评价。总的来说，本研究提供了一种简单、方便、无标记和经济的方法，使用 DNA 粘液构建基于纸质的距离传感器，在各种应用中对 DNase I 的检测具有广阔的前景。
