Hou Jie, Wang Zhan, Li Hong, Zhang Hongzhi, Luo Lan
The People's Hospital of Baoan Shenzhen, The 8th People's Hospital of Shenzhen, Shenzhen, China.
The Affiliated Baoan Hospital of Southern Medical University, Guangzhou, China.
J Oncol. 2022 Jul 6;2022:9332844. doi: 10.1155/2022/9332844. eCollection 2022.
The hepatitis B virus (HBV) infection led to hepatitis, which was one of common reasons for hepatocellular carcinoma (HCC). The immune microenvironment alteration played a crucial role in this process. The study aimed to identify immune-related long noncoding RNAs (lncRNAs) in HBV-related HCC and explore potential mechanisms.
In total, 1,072 immune-related genes (IRGs) were enriched in different co-expression modules with weighted gene co-expression network analysis (WGCNA) combining the corresponding clinical features in HBV-related HCC. The immune-related lncRNAs were selected from the crucial co-expression model based on the correlation analysis with IRGs. The immune-related lncRNAs were furtherly used to construct prognostic signature by the Cox proportional hazards regression and Lasso regression. Furthermore, the proliferation and migration ability of lncRNA SNHG10 were verified in vitro.
A total of nine co-expression modules were identified by WGCNA of which the "red" co-expression module was most correlated with various clinical characteristics. Additionally, the IRGs in this module were significantly enriched in multiple immune-related pathways. The twelve immune-related lncRNAs prognostic signature (HAND2-AS1, LINC00844, SNHG10, MALAT1, LINC00460, LBX2-AS1, MIR31HG, SEMA6A-AS1, LINC1278, LINC00514, CTBP-AS2, and LINC00205) was constructed. The risk score was an independent risk factor in HBV-related HCC and verified by principal components analysis (PCA), nomogram, and PCR between different cell lines. Moreover, the proportion of immune cells were significantly different between high-risk score group and low-risk score group. The malignant behavior of Hep3B was significantly different between si-lncRNA SNHG10 and control group.
The immune-related lncRNAs prognostic signature provided some potential biomarkers and molecular mechanisms in HBV-related HCC.
乙型肝炎病毒（HBV）感染可导致肝炎，这是肝细胞癌（HCC）的常见病因之一。免疫微环境改变在此过程中起关键作用。本研究旨在鉴定HBV相关HCC中免疫相关的长链非编码RNA（lncRNA）并探索潜在机制。
通过加权基因共表达网络分析（WGCNA）结合HBV相关HCC的相应临床特征，在不同共表达模块中富集了总共1072个免疫相关基因（IRG）。基于与IRG的相关性分析，从关键共表达模型中选择免疫相关lncRNA。通过Cox比例风险回归和Lasso回归进一步使用免疫相关lncRNA构建预后特征。此外，在体外验证了lncRNA SNHG10的增殖和迁移能力。
通过WGCNA共鉴定出9个共表达模块，其中“红色”共表达模块与各种临床特征相关性最高。此外，该模块中的IRG在多个免疫相关途径中显著富集。构建了包含12个免疫相关lncRNA的预后特征（HAND2-AS1、LINC00844、SNHG10、MALAT1、LINC00460、LBX2-AS1、MIR31HG、SEMA6A-AS1、LINC1278、LINC00514、CTBP-AS2和LINC00205）。风险评分是HBV相关HCC的独立危险因素，并通过主成分分析（PCA）、列线图和不同细胞系之间的PCR进行了验证。此外，高风险评分组和低风险评分组之间免疫细胞比例存在显著差异。si-lncRNA SNHG10组和对照组之间Hep3B的恶性行为存在显著差异。
免疫相关lncRNA预后特征为HBV相关HCC提供了一些潜在的生物标志物和分子机制。
