男性 HIV 感染者和未感染者免疫细胞衰老的影响。

Effect of aging on immune cells in male HIV-infected and -uninfected healthy individuals.

机构信息

Laboratory of Immunoregulation.

NIAID Collaborative Bioinformatics Resource (NCBR), National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.

出版信息

AIDS. 2022 Nov 15;36(14):1935-1940. doi: 10.1097/QAD.0000000000003331. Epub 2022 Jul 16.

DOI:10.1097/QAD.0000000000003331

PMID:35848591

Abstract

OBJECTIVE

HIV induces immunologic dysfunction in T cells of infected individuals. However, the impact of aging on T cell phenotypes in HIV-infected individuals receiving antiretroviral therapy (ART) has not been fully delineated. We evaluated the relationship between aging and the expression of immune activation and exhaustion markers on CD8 + T cells of age-matched HIV-infected and -uninfected male participants.

DESIGN

Levels of immune activation and exhaustion markers on peripheral blood CD8 + T cells of HIV-infected and -uninfected participants were examined.

METHODS

110 HIV-infected aviremic male participants receiving ART and 146 HIV-uninfected male participants were studied. The levels of TIGIT, PD-1, CD38, and CD226 on CD8 + T cells of the study participants were determined by flow cytometry.

RESULTS

The level of TIGIT on CD8 + T cells was higher in aviremic HIV-infected compared to uninfected participants ( P < 0.0001). In contrast, no significant differences were found in the levels of PD-1 and CD38 on CD8 + T cells between the two groups. Statistically significant correlations were observed between age and the levels of TIGIT + and CD38 + CD8 + T cells in both groups; however, no correlation was found between age and the level of PD-1 + CD8 + T cells in HIV-infected participants. Age-stratification of HIV-infected and -uninfected groups did not show any significant differences in the level of PD-1 expression on CD8 + T cells.

CONCLUSIONS

The findings of our study highlight the role of aging in the expression of immune markers on CD8 + T cells and have important implications for therapies that target immune checkpoints in HIV-infected individuals.

摘要

目的

HIV 可诱导感染个体的 T 细胞免疫功能障碍。然而，在接受抗逆转录病毒疗法（ART）的 HIV 感染者中，衰老对 T 细胞表型的影响尚未完全阐明。我们评估了年龄与 HIV 感染和未感染男性参与者外周血 CD8+T 细胞上免疫激活和耗竭标志物表达之间的关系。

设计

检查了 HIV 感染和未感染参与者外周血 CD8+T 细胞上免疫激活和耗竭标志物的水平。

方法

研究了 110 名接受 ART 的 HIV 感染且病毒载量抑制的男性参与者和 146 名 HIV 未感染的男性参与者。通过流式细胞术测定研究参与者 CD8+T 细胞上 TIGIT、PD-1、CD38 和 CD226 的水平。

结果

与未感染的参与者相比，病毒载量抑制的 HIV 感染者的 CD8+T 细胞上 TIGIT 的水平更高（P<0.0001）。相比之下，两组间 CD8+T 细胞上 PD-1 和 CD38 的水平没有差异。在两组中，年龄与 TIGIT+和 CD38+CD8+T 细胞的水平之间均观察到统计学显著的相关性；然而，在 HIV 感染者中，年龄与 PD-1+CD8+T 细胞的水平之间未发现相关性。对 HIV 感染和未感染组进行年龄分层，CD8+T 细胞上 PD-1 表达的水平没有差异。