评估.的植物毒性、遗传毒性和酶抑制潜力。
Assessment of phytotoxic, genotoxic and enzyme inhibition potential of.
机构信息
Department of Pharmacy, Abasyn University Peshawar, Peshawar 25000, Khyber Pakhtunkhwa, Pakistan.
Department of Pharmacy, University of Peshawar, Peshawar 25120, Khyber Pakhtunkhwa, Pakistan.
出版信息
J Tradit Chin Med. 2022 Aug;42(4):595-603. doi: 10.19852/j.cnki.jtcm.2022.04.005.
OBJECTIVE
To evaluate Sterculia diversifolia stem bark and leaves for phytotoxic, genotoxic and enzymes inhibition potential.
METHODS
Phytotoxic activity of both stem bark and leaves were screened using Lemna minor. The genotoxic activity of Sterculia diversifolia stem bark and leaves extracts were tested using comet assay protocol while enzyme inhibition activity of crude extract and various fractions of both stem bark and leaves were evaluated using acetyl cholinesterase, lipoxygenase, β-glu-curonidase, urease, xanthine oxidase and carbonic anhydrase.
RESULTS
Phytotoxic activity showed significant results in dose dependant manner in both stem bark (ethyl acetate and n-butanol) and leaves (ethyl acetate, n-butanol and n-hexane) fractions. In genotoxic activity, dichloromethane fraction showed significant activity followed by ethyl acetate fraction. Acetyl cholinesterease inhibitory activity showed significant results in both stem bark and leaves fractions, while significant lipoxygenase inhibition was shown by ethyl acetate, dichloromethane, crude extract and n-hexane fractions of both stem bark and leaves. β-glucuronidase, urease and carbonic anhydrase inhibitory activity showed highly significant results in ethyl acetate fraction of both stem bark and leaves, while xanthine oxidase inhibition was shown by dichloromethane fraction of stem bark and leaves extracts.
CONCLUSIONS
This study emphasizes the important phytotoxic, genotoxic and enzyme inhibition effects of Sterculia diversifolia stem bark and leaves. Hence, it is clear that Sterculia diversifolia stem bark and leaves possess phytotoxic, genotoxic and enzyme inhibitory agents.
目的
评估海杧果茎皮和叶的植物毒性、遗传毒性和酶抑制潜力。
方法
使用浮萍筛选海杧果茎皮和叶的植物毒性。采用彗星试验方案测试海杧果茎皮和叶提取物的遗传毒性，同时评估粗提取物和茎皮及叶各部分的不同馏分对乙酰胆碱酯酶、脂氧合酶、β-葡萄糖醛酸酶、脲酶、黄嘌呤氧化酶和碳酸酐酶的抑制活性。
结果
海杧果茎皮（乙酸乙酯和正丁醇）和叶（乙酸乙酯、正丁醇和正己烷）馏分均显示出显著的剂量依赖性植物毒性活性。在遗传毒性活性中，二氯甲烷馏分显示出显著的活性，其次是乙酸乙酯馏分。乙酰胆碱酯酶抑制活性在茎皮和叶各馏分中均显示出显著的结果，而脂氧合酶的显著抑制作用则由乙酸乙酯、二氯甲烷、粗提取物和海杧果茎皮及叶的正己烷馏分显示。β-葡萄糖醛酸酶、脲酶和碳酸酐酶抑制活性在茎皮和叶的乙酸乙酯馏分中表现出高度显著的结果，而黄嘌呤氧化酶抑制作用则由茎皮和叶提取物的二氯甲烷馏分显示。
结论
本研究强调了海杧果茎皮和叶具有重要的植物毒性、遗传毒性和酶抑制作用。因此，很明显，海杧果茎皮和叶含有植物毒性、遗传毒性和酶抑制物质。
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