Ding Jiadong, Su Guannan, Wang Huafei, Tian Xin, Xu Jing, Li Na, Luo Xiang, Yang Peizeng
Department of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, Henan Province Eye Hospital, Henan International Joint Research Laboratory for Ocular Immunology and Retinal Injury Repair, Zhengzhou, People's Republic of China; The Academy of Medical Sciences, Zhengzhou University, Zhengzhou, China.
The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing Branch of National Clinical Research Center for Ocular Diseases, Chongqing, China.
Clin Exp Ophthalmol. 2022 Dec;50(9):1065-1081. doi: 10.1111/ceo.14138. Epub 2022 Aug 12.
To investigate and compare the metabolic profiles in the aqueous humour of Han Chinese patients with Fuchs' syndrome and presumed viral-induced anterior uveitis (PVIAU).
The metabolites in the aqueous humour of 20 Fuchs' syndrome patients, 20 PVIAU patients and 20 senile cataract control patients were detected by liquid chromatography with mass spectrometry. Differential metabolites were analysed by Student's t test, multivariate analysis, cluster analysis and correlation analysis. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis was applied to explore the potential disrupted metabolic pathways in Fuchs' syndrome and PVIAU.
Comparisons of metabolic profiles identified 29 differential metabolites between Fuchs' syndrome patients and controls, 36 differential metabolites between PVIAU patients and controls, and 30 differential metabolites between Fuchs' syndrome patients and PVIAU patients. DL-serine was markedly elevated in Fuchs' syndrome, and 1-palmitoyl-sn-glycero-3-phosphocholine in PVIAU. KEGG pathway analysis suggested that the differential metabolites in Fuchs' syndrome compared with control were mostly enriched in central carbon metabolism in cancer, adenosine triphosphate-binding cassette (ABC) transporters and mineral absorption, while those in PVIAU compared with control were mostly enriched in protein digestion and absorption, biosynthesis of unsaturated fatty acids, and ABC transporters. The metabolic pathways differentially affected in Fuchs' syndrome compared to PVIAU included central carbon metabolism in cancer, protein digestion and absorption and ascorbate and aldarate metabolism.
In Fuchs' syndrome and PVIAU patients, the aqueous humour exhibited specific metabolic profiles and enriched metabolic pathways, which provides a better understanding of the pathogenesis of Fuchs' syndrome and PVIAU in Han Chinese patients.
研究并比较汉族Fuchs综合征患者与疑似病毒感染性前葡萄膜炎（PVIAU）患者房水中的代谢谱。
采用液相色谱-质谱联用技术检测20例Fuchs综合征患者、20例PVIAU患者和20例老年性白内障对照患者房水中的代谢物。通过学生t检验、多变量分析、聚类分析和相关性分析对差异代谢物进行分析。应用京都基因与基因组百科全书（KEGG）分析来探索Fuchs综合征和PVIAU中潜在的代谢途径紊乱情况。
代谢谱比较发现，Fuchs综合征患者与对照之间有29种差异代谢物，PVIAU患者与对照之间有36种差异代谢物，Fuchs综合征患者与PVIAU患者之间有30种差异代谢物。Fuchs综合征中DL-丝氨酸显著升高，PVIAU中1-棕榈酰-sn-甘油-3-磷酸胆碱显著升高。KEGG通路分析表明，与对照相比，Fuchs综合征中的差异代谢物大多富集于癌症中的中心碳代谢、三磷酸腺苷结合盒（ABC）转运体和矿物质吸收，而与对照相比，PVIAU中的差异代谢物大多富集于蛋白质消化与吸收、不饱和脂肪酸生物合成和ABC转运体。与PVIAU相比，Fuchs综合征中受差异影响的代谢途径包括癌症中的中心碳代谢、蛋白质消化与吸收以及抗坏血酸和醛糖代谢。
在Fuchs综合征和PVIAU患者中，房水呈现出特定的代谢谱和富集的代谢途径，这有助于更好地理解汉族患者中Fuchs综合征和PVIAU的发病机制。
