Department of Stomotology, Tianjin Medical University Second Hospital, 23 Pingjiang Road, Hexi District, Tianjin, 300211, China.
School and Hospital of Stomatology, Tianjin Medical University, 12 Observatory Road, Tianjin, 030070, China.
Biomater Sci. 2022 Aug 24;10(17):4773-4784. doi: 10.1039/d2bm00725h.
Titanium (Ti) and its alloys have been universally used as surgical implants, and the clinical need for modifying titanium surfaces to accelerate early stage osseointegration and prevent implant loosening is in huge demand. 3D printing technology is an accurate and controllable method to create titanium implants with complex nanostructures, which provide enough space to react and fit in the microenvironment of cells. Recently, extracellular vesicles (EVs) have attracted attention in promoting osteogenesis. The vesicles derived from bone marrow mesenchymal stem cells (BMSC-EVs) have been proved to pack osteogenic-relative RNAs thereby regulating the osteogenic differentiation and mineralization of the target BMSCs. Arg-Gly-Asp (RGD)-derived peptides are typical peptides used to improve cell attachment and proliferation in bone tissue engineering. A novel strategy is proposed to load RGD-derived peptides on EVs with a fusion peptide (EVs) and colonize EVs on the titanium surface a specific bonding peptide. In this study, we verify that the presence of EVs enables the realization of the synergetic effect of EVs and RGD, enhancing the osteogenic differentiation and mineralization of BMSCs , resulting in satisfactory osseointegration around implants .
钛（Ti）及其合金已被广泛用作外科植入物，临床需要修饰钛表面以加速早期骨整合并防止植入物松动的需求巨大。3D 打印技术是一种精确且可控的方法，可以用复杂的纳米结构来制造钛植入物，为细胞的微环境提供足够的空间来反应和适应。最近，细胞外囊泡（EVs）在促进成骨方面引起了关注。源自骨髓间充质干细胞（BMSC-EVs）的囊泡已被证明可以包装与成骨相关的 RNA，从而调节靶 BMSC 的成骨分化和矿化。精氨酸-甘氨酸-天冬氨酸（RGD）衍生肽是一种用于改善骨组织工程中细胞附着和增殖的典型肽。提出了一种新策略，即将 RGD 衍生肽加载到具有融合肽（EVs）的 EVs 上，并将 EVs 定植在钛表面的特定结合肽上。在这项研究中，我们验证了 EVs 的存在能够实现 EVs 和 RGD 的协同效应，增强 BMSC 的成骨分化和矿化，从而在植入物周围实现令人满意的骨整合。
