Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education & National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.
College of Agriculture, Shanxi Agricultural University, Taiyuan 030031, Shanxi, China.
Genome. 2022 Aug 1;65(8):443-457. doi: 10.1139/gen-2022-0003. Epub 2022 Jul 18.
is an important non-timber tree species for producing high-value agarwood, which is widely used as a traditional medicine and incense. Agarwood is the product of trees in response to injury and fungal infection. The APETALA2/ethylene responsive factor () transcription factors (TFs) play important roles in plant stress responses and metabolite biosynthesis. In this study, 119 genes were identified from the genome and divided into ERF, AP2, RAV, and Soloist subfamilies. Their conserved motif, gene structure, chromosomal localization, and subcellular localization were characterized. A stress/defense-related ERF-associated amphiphilic repression (EAR) motif and an EDLL motif were identified. Moreover, 11 genes that were highly expressed in the agarwood layer in response to whole-tree agarwood induction technique (Agar-Wit) treatment were chosen, and their expression levels in response to methyl jasmonate (MeJA), salicylic acid (SA), or salt treatment were further analyzed using the quantitative real time PCR (qRT-PCR). Among the 11 genes, eight belonged to subgroup B-3. All 11 genes were significantly upregulated under salt treatment, while eight genes were significantly induced by both MeJA and SA. In addition, the gene clusters containing these upregulated genes on chromosomes were observed. The results obtained from this research not only provide useful information for understanding the functions of genes in but also identify candidate genes and gene clusters to dissect their regulatory roles in agarwood formation for future research.
是生产高价值沉香的重要非木材树种，广泛用作传统药物和香料。沉香是树木对损伤和真菌感染的反应产物。APETALA2/乙烯响应因子（AP2/ERF）转录因子（TFs）在植物应激反应和代谢物生物合成中发挥重要作用。在这项研究中，从 基因组中鉴定出 119 个基因，并分为 ERF、AP2、RAV 和 Soloist 亚家族。它们的保守基序、基因结构、染色体定位和亚细胞定位得到了表征。鉴定出一个与应激/防御相关的 ERF 相关的两亲性抑制（EAR）基序和一个 EDLL 基序。此外，选择了 11 个在整株沉香诱导技术（Agar-Wit）处理后对沉香层高度表达的基因，并进一步使用定量实时 PCR（qRT-PCR）分析它们对茉莉酸甲酯（MeJA）、水杨酸（SA）或盐处理的表达水平。在这 11 个基因中，有 8 个属于 B-3 亚组。所有 11 个基因在盐处理下均显著上调，而 8 个基因同时被 MeJA 和 SA 显著诱导。此外，还观察到这些上调基因在染色体上的基因簇。本研究的结果不仅为了解 基因在 中的功能提供了有用信息，还鉴定了候选基因和基因簇，以剖析它们在沉香形成中的调控作用，为未来的研究提供了依据。
