Chavez D J, McIntyre J A, Colliver J A, Faulk W P
J Immunol. 1987 Jul 1;139(1):85-8.
CBA/J female mice have a high rate of spontaneous fetal loss when mated with DBA/2 males. We have confirmed that this fetal resorption rate can be significantly reduced by immunizing the female with C57BL rather than DBA leukocytes. The current studies have been extended to show the effect of continued immunization into second pregnancies. Three new findings emerge: second pregnancies in unimmunized CBA/J females X DBA/2 males proceed with a low percentage of spontaneous resorptions; continued immunization of multigravid, multiparous mice is associated with a high percentage of late onset fetal resorptions; and comparison of sex ratios between treated and untreated pregnancies showed no significant shift. These results suggest that untreated CBA/J females mated to DBA/2 males can be immunized to trophoblast antigens during first pregnancies, and that such immunization leads to "normal" reproductive outcome in subsequent untreated pregnancies. Persistent immunization with leukocytes appears to increase the percentage of fetal wastage in subsequent pregnancies regardless of whether the mother receives paternal or nonpaternal leukocytes. Intentional chronic immunization with male leukocytes does not influence the sex ratio of viable offspring. These data are discussed with reference to immunotherapy for women who suffer unexplained, recurrent spontaneous abortions.
CBA/J 雌性小鼠与 DBA/2 雄性小鼠交配时，自然流产率很高。我们已经证实，用 C57BL 而非 DBA 白细胞免疫雌性小鼠，可以显著降低这种胚胎吸收的发生率。目前的研究已经扩展到显示持续免疫至第二次怀孕的效果。出现了三个新发现：未免疫的 CBA/J 雌性小鼠与 DBA/2 雄性小鼠第二次怀孕时，自然吸收的比例较低；多次怀孕、多产的小鼠持续免疫与高比例的晚期胚胎吸收有关；比较经处理和未经处理的怀孕之间的性别比例，未发现显著变化。这些结果表明，与 DBA/2 雄性小鼠交配的未处理 CBA/J 雌性小鼠在第一次怀孕时可被免疫滋养层抗原，并且这种免疫会导致随后未处理怀孕的“正常”生殖结果。无论母亲接受的是父系还是非父系白细胞，用白细胞持续免疫似乎都会增加随后怀孕时胚胎丢失的比例。用雄性白细胞进行有意的慢性免疫不会影响存活后代的性别比例。我们参考了对患有不明原因反复自然流产的女性的免疫疗法来讨论这些数据。
