sp. nov., isolated from the rhizosphere soil of L.

A novel actinobacterium, designated strain SF1, was isolated from the rhizosphere soil of a star fruit plant ( L.) collected from Bangkok, Thailand, and its taxonomic position was evaluated. The strain showed morphological, chemotaxonomic and phylogenetic characteristics consistent with its classification in the genus . Strain SF1 was an aerobic, Gram-stain-positive and non-motile actinobacterium. Growth occurred at 15-35 °C, at pH 4.0-12.0 and in the presence of 0-10 % (w/v) NaCl. The 16S rRNA gene sequence of strain SF1 showed the highest similarity to YIM 0006 (99.5 %), 014-5 (98.8 %) and A-T 8314 (98.8 %). The genome sequencing revealed a genome size of 6.52 Mbp and a DNA G+C content of 74.0 %. In addition, the average nucleotide identity values between strain SF1 and reference strains, DSM 45062, DSM 45772 and TBRC 5722, were found to be 86.1, 86.5 and 79.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 32.4, 32.4 and 23.3 %, respectively. The whole-cell hydrolysates of strain SF1 contained -diaminopimelic acid as the diagnostic diamino acid, with arabinose, galactose, glucose and ribose as whole-cell sugars. The predominant menaquinone was MK-8(H). Major cellular fatty acids were iso-C and a summed feature consisting of C ω6/C ω7. Characterization based on chemotaxonomic, phylogenetic, phenotypic and genomic evidence demonstrated that strain SF1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain SF1 (=TBRC 15166= NBRC 115295).