Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
Biodiversity Center Kasetsart University (BDCKU), Bangkok 10900, Thailand.
Int J Syst Evol Microbiol. 2022 Jul;72(7). doi: 10.1099/ijsem.0.005277.
A novel actinobacterium, designated strain SF1, was isolated from the rhizosphere soil of a star fruit plant ( L.) collected from Bangkok, Thailand, and its taxonomic position was evaluated. The strain showed morphological, chemotaxonomic and phylogenetic characteristics consistent with its classification in the genus . Strain SF1 was an aerobic, Gram-stain-positive and non-motile actinobacterium. Growth occurred at 15-35 °C, at pH 4.0-12.0 and in the presence of 0-10 % (w/v) NaCl. The 16S rRNA gene sequence of strain SF1 showed the highest similarity to YIM 0006 (99.5 %), 014-5 (98.8 %) and A-T 8314 (98.8 %). The genome sequencing revealed a genome size of 6.52 Mbp and a DNA G+C content of 74.0 %. In addition, the average nucleotide identity values between strain SF1 and reference strains, DSM 45062, DSM 45772 and TBRC 5722, were found to be 86.1, 86.5 and 79.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 32.4, 32.4 and 23.3 %, respectively. The whole-cell hydrolysates of strain SF1 contained -diaminopimelic acid as the diagnostic diamino acid, with arabinose, galactose, glucose and ribose as whole-cell sugars. The predominant menaquinone was MK-8(H). Major cellular fatty acids were iso-C and a summed feature consisting of C ω6/C ω7. Characterization based on chemotaxonomic, phylogenetic, phenotypic and genomic evidence demonstrated that strain SF1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain SF1 (=TBRC 15166= NBRC 115295).
一株新的放线菌，菌株 SF1，从一株来自泰国曼谷的杨桃植物的根际土壤中分离出来，并对其分类地位进行了评估。该菌株的形态学、化学生态学和系统发育特征与其在属中的分类一致。菌株 SF1 是一种需氧、革兰氏阳性、非运动性放线菌。生长温度为 15-35°C，pH 值为 4.0-12.0，盐度为 0-10%（w/v）。菌株 SF1 的 16S rRNA 基因序列与 YIM 0006（99.5%）、014-5（98.8%）和 A-T 8314（98.8%）的相似度最高。基因组测序显示其基因组大小为 6.52 Mbp，GC 含量为 74.0%。此外，菌株 SF1 与参考菌株 DSM 45062、DSM 45772 和 TBRC 5722 的平均核苷酸同一性值分别为 86.1%、86.5%和 79.7%，它们之间的数字 DNA-DNA 杂交水平分别为 32.4%、32.4%和 23.3%。菌株 SF1 的全细胞水解物含有 -二氨基庚二酸作为诊断二氨基庚二酸，全细胞糖含有阿拉伯糖、半乳糖、葡萄糖和核糖。主要的类异戊二烯醌是 MK-8(H)。主要的细胞脂肪酸是 iso-C 和一个由 Cω6/Cω7 组成的总和特征。基于化学分类学、系统发育、表型和基因组证据的特征表明，菌株 SF1 代表了属的一个新种，建议将其命名为 sp. nov.。模式菌株为 SF1 菌株（=TBRC 15166=NBRC 115295）。
