sp. nov., an actinobacterium isolated from rice rhizosphere soil in Thailand.

A novel actinomycete, designated strain RS11V-5, was isolated from rhizosphere soil of L. collected from Roi Et Province, Thailand, and its taxonomic position was evaluated. Cells of strain RS11V-5 were Gram-stain-positive, aerobic, and non-motile. Whole-cell hydrolysates contained -diaminopimelic acid, arabinose, galactose, glucose and ribose. MK-8(H) was detected as the predominant menaquinone of this strain. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylmethylethanolamine, hydroxy-phosphatidylethanolamine, an unidentified phospholipid, an unidentified aminolipid and an unidentified glycolipid. The major fatty acids were iso-C, C and C ω7/C ω6. Phylogenetic analyses based on the 16S rRNA gene sequences showed that strain RS11V-5 belonged to the genus and had high 16S rRNA sequence similarity of 99.3 % to KCTC 29062 and less than 98.4 % to other members of the genus . The DNA G+C content of the strain RS11V-5 was 73.3 mol%. Strain RS11V-5 showed 46.5 % digital DNA-DNA hybridization, 92.2 % orthologous average nucleotide identity (OrthoANI), 90.2 % ANI based on blast and 92.7 % ANI based on MUMmer to KCTC 29062. Based its phenotypic, genotypic, phylogenetic and chemotaxonomic characteristics, strain RS11V-5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RS11V-5 (=TBRC 15286=NBRC 115296).