Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
Biodiversity Center Kasetsart University (BDCKU), Bangkok 10900, Thailand.
Int J Syst Evol Microbiol. 2022 Jun;72(6). doi: 10.1099/ijsem.0.005430.
A novel actinomycete, designated strain RS11V-5, was isolated from rhizosphere soil of L. collected from Roi Et Province, Thailand, and its taxonomic position was evaluated. Cells of strain RS11V-5 were Gram-stain-positive, aerobic, and non-motile. Whole-cell hydrolysates contained -diaminopimelic acid, arabinose, galactose, glucose and ribose. MK-8(H) was detected as the predominant menaquinone of this strain. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylmethylethanolamine, hydroxy-phosphatidylethanolamine, an unidentified phospholipid, an unidentified aminolipid and an unidentified glycolipid. The major fatty acids were iso-C, C and C ω7/C ω6. Phylogenetic analyses based on the 16S rRNA gene sequences showed that strain RS11V-5 belonged to the genus and had high 16S rRNA sequence similarity of 99.3 % to KCTC 29062 and less than 98.4 % to other members of the genus . The DNA G+C content of the strain RS11V-5 was 73.3 mol%. Strain RS11V-5 showed 46.5 % digital DNA-DNA hybridization, 92.2 % orthologous average nucleotide identity (OrthoANI), 90.2 % ANI based on blast and 92.7 % ANI based on MUMmer to KCTC 29062. Based its phenotypic, genotypic, phylogenetic and chemotaxonomic characteristics, strain RS11V-5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RS11V-5 (=TBRC 15286=NBRC 115296).
一株新的放线菌，命名为 RS11V-5 菌株，从泰国罗勇府采集的 L. 的根际土壤中分离得到，并对其分类地位进行了评估。RS11V-5 菌株的细胞革兰氏阳性，需氧，不运动。全细胞水解物含有二氨基庚二酸、阿拉伯糖、半乳糖、葡萄糖和核糖。该菌株的主要类异戊二烯醌为 MK-8(H)。极性脂类包括双磷脂酰甘油、磷脂酰甲乙醇胺、磷脂乙醇胺、羟基磷脂甲乙醇胺、羟基磷脂乙醇胺、一种未鉴定的磷脂、一种未鉴定的氨基脂和一种未鉴定的糖脂。主要脂肪酸为 iso-C、C 和 C ω7/C ω6。基于 16S rRNA 基因序列的系统发育分析表明，RS11V-5 菌株属于 属，与 KCTC 29062 的 16S rRNA 序列相似性高达 99.3 %，而与属内其他成员的相似性低于 98.4 %。该菌株 RS11V-5 的 DNA G+C 含量为 73.3 mol%。RS11V-5 菌株的数字 DNA-DNA 杂交率为 46.5 %，与 KCTC 29062 的同源平均核苷酸同一性（OrthoANI）为 92.2 %，基于 blast 的ANI 为 90.2 %，基于 MUMmer 的 ANI 为 92.7 %。基于表型、基因型、系统发育和化学分类特征，RS11V-5 菌株代表了 属的一个新种，建议命名为 sp. nov.。模式菌株为 RS11V-5（=TBRC 15286=NBRC 115296）。
