Cao Nan, Liu Xudong, Tang Xuechun, Gao Ruijuan, Ma Ketao, Li Li, Si Junqiang
Key Laboratory of Xinjiang Local and Ethnic High Incidence, Ministry of Education, School of Medicine, Shihezi University, Shihezi 832000; Department of Physiology, School of Medicine, Shihezi University, Shihezi 832000; NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases, Shihezi 832002, China.
Department of Radiology and CT, People's Hospital of Changji Prefecture, Changji 831000, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2022 Aug;38(8):707-713.
Objective To investigate the effect of emodin on the proliferation and migration of human pulmonary artery smooth muscle cells (HPASMCs) induced by transforming growth factor β1 (TGF-β1). Methods HPASMCs were cultured in vitro, and HPASMCs in logarithmic growth phase were divided into control group, TGF-β1 group, and TGF-β1 combined with emodin group. The activity of HPASMCs was detected by CCK-8 assay, the migration ability of HPASMCs was detected by Transwell chamber assay and scratch assay, and the expressions of osteopontin (OPN) and proliferating cell nuclear antigen (PCNA) were detected by immunofluorescence assay. The protein levels of OPN and PCNA and the phosphorylation of SMAD family member 2 (SMAD2) and SMAD family member 2 (SMAD3) in HPASMCs were detected by Western blot. Results Compared with those in the control group, in TGF-β1 group, the protein expressions of OPN and PCNA, the proliferation and migration of HPASMCs, and the phosphorylation of SMAD2 and SMAD3 were increased. Compared with those in the TGF-β1 group, in the TGF-β1 combined with emodin group, the proliferation and migration of HPASMCs, the expressions of OPN and PCNA, and the phosphorylation of SMAD2 and SMAD3 were decreased. Conclusion Emodin inhibits the up-regulation of OPN and PCNA and the proliferation and migration of PASMCs induced by TGF-β1, which may be related to the blocking of SMAD2/3 signaling pathway.
目的 探讨大黄素对转化生长因子β1（TGF-β1）诱导的人肺动脉平滑肌细胞（HPASMCs）增殖和迁移的影响。方法 体外培养HPASMCs，将对数生长期的HPASMCs分为对照组、TGF-β1组和TGF-β1联合大黄素组。采用CCK-8法检测HPASMCs活性，采用Transwell小室法和划痕法检测HPASMCs迁移能力，采用免疫荧光法检测骨桥蛋白（OPN）和增殖细胞核抗原（PCNA）的表达。采用蛋白质印迹法检测HPASMCs中OPN和PCNA的蛋白水平以及SMAD家族成员2（SMAD2）和SMAD家族成员3（SMAD3）的磷酸化水平。结果 与对照组相比，TGF-β1组中OPN和PCNA的蛋白表达、HPASMCs的增殖和迁移以及SMAD2和SMAD3的磷酸化水平均升高。与TGF-β1组相比，TGF-β1联合大黄素组中HPASMCs的增殖和迁移、OPN和PCNA的表达以及SMAD2和SMAD3的磷酸化水平均降低。结论 大黄素抑制TGF-β1诱导的OPN和PCNA上调以及PASMCs的增殖和迁移，这可能与阻断SMAD2/3信号通路有关。
