Tang Ming, Yang Jie, Yuan Qing, Xu Wenfeng, Xie Xiang, Yu Hong
The School of Basic Medical Science, Southwest Medical University, Luzhou 646000, China.
The School of Basic Medical Science, Southwest Medical University, Luzhou 646000, China.*Corresponding author, E-mail:
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2022 Aug;38(8):727-735.
Objective To explore the clinical significance of inhibitor of NF-κB (IκB) family in lung adenocarcinoma (LUAD) through bioinformatics analysis. Methods The differentially expressed genes of IκB family in LUAD were screened by R language for survival analysis. The correlation between the expression of IκB family genes and clinicopathological characteristics was analyzed by R language, and the genes related to survival rate were selected for further study. GO and KEGG enrichment analyses were performed with LinkedOmics. The infiltration of immune cells was analyzed with TIMER. The correlation between the candidate genes and the prognosis of LUAD was analyzed through COX model. Results The expression levels of NF-κB inhibitor δ (NFKBID) and NF-κB inhibitor Zeta (NFKBIZ) were significantly downregulated in tumor tissues, and the patients with low expression levels of NFKBID and NFKBIZ had shorter overall survival. NFKBID and NFKBIZ were significantly correlated with T stage of LUAD. Enrichment analysis showed that low expression levels of NFKBID and NFKBIZ were correlated with energy metabolism and protein expression and transport. The expression levels of NFKBID and NFKBIZ were positively correlated with the infiltration of B cells, CD4 T cells, neutrophils, and dendritic cells. COX analysis indicated that NFKBIZ could be an independent prognostic factor for LUAD. Conclusion The expression levels of NFKBID and NFKBIZ were significantly downregulated in tumor tissues, and were correlated with overall survival. NFKBID and NFKBIZ could be involved in the occurrence and development of LUAD by regulating glycometabolism and multiple immune cells infiltration. NFKBIZ could be considered as an independent prognostic factor for LUAD.
目的 通过生物信息学分析探讨核因子κB抑制蛋白（IκB）家族在肺腺癌（LUAD）中的临床意义。方法 用R语言筛选LUAD中IκB家族的差异表达基因进行生存分析。用R语言分析IκB家族基因表达与临床病理特征的相关性，选择与生存率相关的基因进行进一步研究。使用LinkedOmics进行基因本体（GO）和京都基因与基因组百科全书（KEGG）富集分析。用肿瘤免疫估计资源（TIMER）分析免疫细胞浸润情况。通过COX模型分析候选基因与LUAD预后的相关性。结果 肿瘤坏死因子α诱导蛋白3（TNFAIP3）和核因子κB抑制蛋白ζ（NFKBIZ）在肿瘤组织中的表达水平显著下调，TNFAIP3和NFKBIZ表达水平低的患者总生存期较短。TNFAIP3和NFKBIZ与LUAD的T分期显著相关。富集分析表明，TNFAIP3和NFKBIZ低表达与能量代谢、蛋白质表达和转运相关。TNFAIP3和NFKBIZ的表达水平与B细胞、CD4 + T细胞、中性粒细胞和树突状细胞的浸润呈正相关。COX分析表明，NFKBIZ可能是LUAD的独立预后因素。结论 TNFAIP3和NFKBIZ在肿瘤组织中的表达水平显著下调，且与总生存期相关。TNFAIP3和NFKBIZ可能通过调节糖代谢和多种免疫细胞浸润参与LUAD的发生发展。NFKBIZ可被视为LUAD的独立预后因素。
