Gitel S N, Medina V M, Wessler S
J Lab Clin Med. 1987 Jun;109(6):672-8.
Antibodies that recognize unmodified heparin bound to positively charged macromolecules have been obtained by immunizing rabbits with a methylated bovine serum albumin-heparin precipitate. These antibodies have been used to develop an immunoassay that can quantitate heparin at a concentration of 5 ng/ml in buffer solutions, and at a concentration of 25 ng/ml in plasma without pretreatment of the sample. The specificity of the antibodies is such that they do not recognize other glycosaminoglycans and are able to distinguish among different commercial heparin samples that are otherwise indistinguishable by specific anticoagulant activity or molecular weight distribution.
通过用甲基化牛血清白蛋白 - 肝素沉淀物免疫兔子，已获得识别与带正电荷大分子结合的未修饰肝素的抗体。这些抗体已用于开发一种免疫测定法，该方法可以在缓冲溶液中定量浓度为5 ng/ml的肝素，在未经样品预处理的血浆中定量浓度为25 ng/ml的肝素。这些抗体的特异性使得它们不识别其他糖胺聚糖，并且能够区分不同的商业肝素样品，而这些样品通过特定的抗凝活性或分子量分布是无法区分的。
