Department of Chemical Sciences, Bernal Institute, SSPC - The SFI Pharmaceutical Research Centre, University of Limerick, Limerick, Ireland.
School of Engineering, Stokes Laboratories, Bernal Institute, University of Limerick, Limerick, Ireland.
Drug Deliv Transl Res. 2023 Jan;13(1):308-319. doi: 10.1007/s13346-022-01201-x. Epub 2022 Jul 18.
In the design of injectable antimicrobial dextran-alginate hydrogels, the impact of dextran oxidation and its subsequent changes in molecular weight and the incorporation of glycol chitosan on (i) gel mechanical strength and (ii) the inhibitory profile of an encapsulated bacteriocin, nisin A, are explored. As the degree of oxidation increases, the weight average molecular mass of the dextran decreases, resulting in a reduction in elastic modulus of the gels made. Upon encapsulation of the bacteriocin nisin into the gels, varying the dextran mass/oxidation level allowed the antimicrobial activity against S. aureus to be controlled. Gels made with a higher molecular weight (less oxidised) dextran show a higher initial degree of inhibition while those made with a lower molecular weight (more oxidised) dextran exhibit a more sustained inhibition. Incorporating glycol chitosan into gels composed of dextran with higher masses significantly increased their storage modulus and the gels' initial degree of inhibition.
在可注射型抗菌性葡聚糖-海藻酸钠水凝胶的设计中，研究了葡聚糖氧化及其随后的分子量变化和糖基壳聚糖的加入对（i）凝胶机械强度和（ii）包封的细菌素乳链菌肽 A 的抑制谱的影响。随着氧化程度的增加，葡聚糖的重均分子量降低，导致所制备的凝胶的弹性模量降低。在将细菌素乳链菌肽 A 包封到凝胶中时，通过改变葡聚糖的质量/氧化水平，可以控制其对金黄色葡萄球菌的抗菌活性。用高分子量（较少氧化）葡聚糖制成的凝胶显示出更高的初始抑制程度，而用低分子量（更多氧化）葡聚糖制成的凝胶则表现出更持续的抑制作用。将糖基壳聚糖掺入由较高质量的葡聚糖组成的凝胶中，显著提高了它们的储能模量和凝胶的初始抑制程度。
