Department of Breast Surgery, The First Affiliated Hospital of Jinan University, Jinan University, Guangzhou, China.
Department of Reproductive Medical Center, Guangdong Women and Children Hospital, Guangzhou, China.
Biosci Rep. 2022 Jul 29;42(7). doi: 10.1042/BSR20220645.
In recent years, breast cancer attracts more and more attention because of its high incidence. To explore the molecular functions and mechanisms, we performed RNA sequencing on the tumor tissues and their paired normal tissues from three breast cancer patients. By differential expression analysis, we found 3764 differentially expressed (DE) mRNAs, 5416 DE lncRNAs, and 148 DE circRNAs. Enrichment analysis suggested that the DE lncRNAs and DE circRNAs were enriched in mitochondria and nucleus, which indicated that they may participate in the vital metabolism directly or indirectly, such as fatty acid metabolism. Subsequently, the protein-protein interaction (PPI) network was constructed and we got 8 key proteins, of which the matrix metalloproteinase-9 (MMP9; degree 5) draws our attention. Based on the 38 up-regulated circRNAs and 14 down-regulated circRNAs, we constructed competing endogenous RNA (ceRNA) networks, from which the has-miR-6794-5p has been identified to enriched in the up-regulated network and correlated with the circNFIX directly. At this point, we presented that the circNFIX and MMP9 may play a significant role by regulating fatty acid metabolism in breast cancer.
近年来，乳腺癌的发病率较高，因此越来越受到关注。为了探讨其分子功能和机制，我们对三例乳腺癌患者的肿瘤组织与其配对的正常组织进行了 RNA 测序。通过差异表达分析，我们发现了 3764 个差异表达的 mRNA、5416 个差异表达的 lncRNA 和 148 个差异表达的 circRNA。富集分析表明，差异表达的 lncRNA 和 circRNA 富集在线粒体和细胞核中，这表明它们可能直接或间接地参与重要的代谢过程，如脂肪酸代谢。随后，我们构建了蛋白质-蛋白质相互作用（PPI）网络，得到了 8 个关键蛋白，其中基质金属蛋白酶-9（MMP9；度为 5）引起了我们的注意。基于 38 个上调的 circRNA 和 14 个下调的 circRNA，我们构建了竞争性内源 RNA（ceRNA）网络，其中 hsa-miR-6794-5p 被鉴定为富集在上调网络中，并与 circNFIX 直接相关。此时，我们提出 circNFIX 和 MMP9 可能通过调节乳腺癌中的脂肪酸代谢发挥重要作用。
