Hunan Academy of Agricultural Sciences, Changsha, Hunan, China.
Hunan Vegetables Research Institute, Changsha, Hunan, China.
Microbiol Spectr. 2022 Aug 31;10(4):e0093322. doi: 10.1128/spectrum.00933-22. Epub 2022 Jul 19.
Imbalances of soil available nutrients and soilborne diseases have seriously restricted the productivity of crops and jeopardized food security worldwide. Pyrroloquinoline quinone (PQQ), a redox cofactor in some bacteria involved in glucose metabolism and phosphorus mineralization, could be anticipated to alter soil ecosystems to a certain extent. However, there is limited information on PQQ defending soilborne pathogens and regulating soil main nutrients. Here, a pot experiment based on mono-cropping soils of pepper was conducted to examine the effects of PQQ amendment on reconstructing soil microbial communities and soil nutrients under aerobic/anaerobic conditions comprising three treatments, namely, control, PQQ (aerobic), and FL-PQQ (anaerobic). The results revealed that soil microbial community composition and soil nutrients were distinctly altered by PQQ regimes. Compared to control, PQQ treatment significantly increased the content of soil available phosphorus (AP), while FL_PQQ treatment strongly improved the content of soil available nitrogen (AN). In terms of pathogens, relative to control, both PQQ treatments suppressed the abundances of pathogens, of which FL_PQQ treatment significantly decreased the abundance of the pathotrophic fungal by 64% and the abundance of Fusarium oxysporum by 57%, largely attributed to the increase of organic acid generators (O, ) and potential antagonists (). Structural equation modeling (SEM) showed that PQQ regimes suppressed pathogens by indirectly regulating soil physicochemical properties and microbial communities. Overall, we proposed that PQQ application both in aerobic/anaerobic conditions could improve soil available nutrients and suppress soil pathogens in pepper monocropping soils. The attention to PQQ (pyrroloquinoline quinone) effect on soil nutrients and pathogens was less paid in monocropping soils. However, the underlying microbial interacting mechanism remains unclear. Adopting a novel external bio-additive, the effects of PQQ on soil main nutrients and the pathotrophic fungal under aerobic and anaerobic regimes will be investigated, which would help to improve soil quality health. Our main conclusion was that PQQ would help to remediate monocropping obstacle soils in terms of soil nutrients and soil pathogens by associating with the microbial community, and anaerobic PQQ application more favored amelioration of continuous obstacle soils. These results will benefit the health and sustainable development of pepper production as well as other greenhouse vegetable production.
土壤有效养分失衡和土传病害严重限制了作物的生产力，危及全球粮食安全。吡咯喹啉醌（PQQ）是某些参与葡萄糖代谢和磷矿化的细菌中的氧化还原辅助因子，预计会在一定程度上改变土壤生态系统。然而，关于 PQQ 防御土传病原菌和调节土壤主要养分的信息有限。在这里，进行了一项基于辣椒单作土壤的盆栽实验，以研究 PQQ 改良在有氧/厌氧条件下重建土壤微生物群落和土壤养分的效果，包括三个处理，即对照、PQQ（有氧）和 FL-PQQ（厌氧）。结果表明，PQQ 处理明显改变了土壤微生物群落组成和土壤养分。与对照相比，PQQ 处理显著增加了土壤有效磷（AP）的含量，而 FL-PQQ 处理则强烈提高了土壤有效氮（AN）的含量。就病原菌而言，与对照相比，两种 PQQ 处理均抑制了病原菌的丰度，其中 FL-PQQ 处理使病原真菌的丰度显著降低了 64%，尖孢镰刀菌的丰度降低了 57%，这主要归因于有机酸生成菌（O，）和潜在拮抗菌（）的增加。结构方程模型（SEM）表明，PQQ 处理通过间接调节土壤理化性质和微生物群落来抑制病原菌。总的来说，我们提出在有氧/厌氧条件下应用 PQQ 可以提高辣椒单作土壤中的土壤有效养分并抑制土壤病原菌。在单作土壤中，对 PQQ（吡咯喹啉醌）对土壤养分和病原菌的影响的关注较少。然而，其潜在的微生物相互作用机制尚不清楚。采用新型外源性生物添加剂，研究 PQQ 在有氧和厌氧条件下对土壤主要养分和病原真菌的影响，有助于改善土壤质量健康。我们的主要结论是，通过与微生物群落的关联，PQQ 将有助于修复土壤养分和土壤病原菌方面的单作障碍土壤，而厌氧 PQQ 应用更有利于连续障碍土壤的改良。这些结果将有利于辣椒生产以及其他温室蔬菜生产的健康和可持续发展。
