College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, China.
Hunan Fisheries Science Institute, Changsha, Hunan, China.
Lett Appl Microbiol. 2022 Nov;75(5):1182-1192. doi: 10.1111/lam.13786. Epub 2022 Jul 31.
To analyse the correlation between the intestinal microbiota (IM) and differential development of Quasipaa spinosa across different stages of metamorphosis, we focused on four key developmental periods (15 days post-hatch [dph; Gosner stage, GS23], 60 dph [GS25], 90 dph [GS41] and 150 dph [GS25, GS41, GS42, GS46]) and used 16S rDNA amplicon sequencing. Results showed that IM diversity in Q. spinosa was related to life history. Specifically, there was a significant difference between the aquatic and terrestrial stages, and IM diversity increased with age. Proteobacteria, Bacteroidetes and Firmicutes were the dominant phyla in tadpoles, whereas Bacteroidetes, Proteobacteria, Firmicutes and Fusobacteria were the dominant phyla at the metamorphosis stages. The composition of the IM at different stages and ages varied considerably, but the changes were not substantial among different development stages at 150 dph. This was probably because age has a greater influence than growth factors, and host selective pressure increases with age. This study provides a theoretical reference for subsequent studies on the IM, as well as for the prevention of amphibian endogenous pathogenic microbial diseases.
为分析肠微生物群（IM）与不同变态阶段棘胸蛙差异发育之间的相关性，我们重点关注了四个关键发育时期（孵化后 15 天[Gosner 阶段 23]、60 天[GS25]、90 天[GS41]和 150 天[GS25、GS41、GS46]），并使用 16S rDNA 扩增子测序。结果表明，棘胸蛙的 IM 多样性与生活史有关。具体而言，水生和陆地阶段之间存在显著差异，IM 多样性随年龄增长而增加。变形阶段的优势门为变形菌门、拟杆菌门和厚壁菌门，而在蝌蚪阶段的优势门为拟杆菌门、变形菌门、厚壁菌门和梭杆菌门。不同阶段和年龄的 IM 组成差异很大，但在 150 天的不同发育阶段之间变化不大。这可能是因为年龄的影响大于生长因素，而且宿主的选择压力随年龄的增长而增加。本研究为后续 IM 研究以及预防两栖类内生病原微生物疾病提供了理论参考。
