Lu Yuwan, Zhang Xiaodan, Huang Yuming
Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China.
Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China.
Biosens Bioelectron. 2022 Nov 1;215:114567. doi: 10.1016/j.bios.2022.114567. Epub 2022 Jul 11.
Through VO etching of ZIF-67 and subsequent pyrolysis in an argon flow, the V doped Co@NC (V/Co@NC) with mixed-valence Co(II)/Co(III) and V(III)/V(IV) was successfully obtained. V doping plays an important role in regulating the enzyme-like activity of Co@NC. Specifically, the Co@NC has both oxidase-like activity and peroxidase-mimic activity, while the V/Co@NC possesses the specific oxidase-like activity. Benefiting from the elevated Co level due to electrons transfer from the reduced V(III) to Co and recyclable redox reactions between the Co(III)/Co(II) and V(IV)/V(III) couples, the V/Co@NC displays 4-fold increase in the oxidase-like activity, smaller K (0.18 mM) and larger V (4.01 × 10 M s) toward TMB relative to Co@NC. The origin of V/Co@NC as oxidase mimic is likely attributed to the generation of O and •OH. Different phenolic compounds (PC), like gallic acid, kaempferol, caffeic acid, quercetin, and catechin, have distinct antioxidant capacity, showing a differential inhibiting effect on the V/Co@NC-TMB system. The different PC antioxidants in the V/Co@NC-TMB system lead to unique decrease in the absorbance at 652 nm (A), resulting in a unique absorbance signal response mode. By choosing different combinations of A signals at various time points, multichannel information can be extracted from a single nanozyme for pattern recognition. Based on this, a colorimetric array sensing platform for the identification of PC is established successfully. Furthermore, the constructed sensor array can be used for quantifying and discriminating multiple PC antioxidants.
通过对ZIF-67进行VO蚀刻并随后在氩气流中热解，成功获得了具有混合价态Co(II)/Co(III)和V(III)/V(IV)的V掺杂Co@NC（V/Co@NC）。V掺杂在调节Co@NC的类酶活性方面起着重要作用。具体而言，Co@NC同时具有类氧化酶活性和过氧化物酶模拟活性，而V/Co@NC具有特定的类氧化酶活性。由于从还原的V(III)到Co的电子转移导致Co含量升高以及Co(III)/Co(II)和V(IV)/V(III)电对之间的可循环氧化还原反应，V/Co@NC相对于Co@NC对TMB的类氧化酶活性提高了4倍，K值更小（0.18 mM），V值更大（4.01×10 M s）。V/Co@NC作为氧化酶模拟物的起源可能归因于O和•OH的产生。不同的酚类化合物（PC），如没食子酸、山奈酚、咖啡酸、槲皮素和儿茶素，具有不同的抗氧化能力，对V/Co@NC-TMB系统表现出不同的抑制作用。V/Co@NC-TMB系统中不同的PC抗氧化剂导致652 nm处吸光度（A）独特下降，产生独特的吸光度信号响应模式。通过选择不同时间点A信号的不同组合，可以从单个纳米酶中提取多通道信息用于模式识别。基于此，成功建立了用于识别PC的比色阵列传感平台。此外，构建的传感器阵列可用于定量和区分多种PC抗氧化剂。
