Department of Chemistry and Biochemistry, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, Zagreb, Croatia.
Department of Microbiology and Infectious Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, Zagreb, Croatia.
Res Vet Sci. 2022 Dec 10;151:42-46. doi: 10.1016/j.rvsc.2022.04.015. Epub 2022 Jul 3.
This study investigated the prevalence and antimicrobial susceptibility of enterococci isolated from dogs and cats with urinary tract infections in northwestern Croatia. During this study, the laboratory received 787 urine samples, 651 from dogs and 136 from cats. A total of 260 urine samples (211 from dogs and 49 from cats) were bacteriologically positive. Of these, 29 isolates belonged to Enterococcus spp.; 22 from dogs and seven from cats. Enterococci isolates were identified by PCR method, 12 of which were Enterococcus faecium and 17 were Enterococcus faecalis species. In dogs, 16 E. faecalis and six E. faecium strains were identified, whereas in cats, six E. faecium and only one E. faecalis strain were identified. Antimicrobial susceptibility was determined by the Kirby-Bauer disk diffusion method for nine antimicrobials: penicillin, ampicillin, vancomycin, nitrofurantoin, rifampicin, tetracycline, chloramphenicol, enrofloxacin, ciprofloxacin. The isolates were tested for high-level resistance to streptomycin and gentamicin. The highest resistance of Enterococcus spp. was observed to rifampicin (86%) and enrofloxacin (83%), followed by tetracycline and ciprofloxacin (69%). Resistance to vancomycin was 28%, and the lowest resistance was to chloramphenicol (17%). Multidrug resistance was found in 76% of enterococci isolates. High-level streptomycin resistance was detected in 17% and high-level gentamicin resistance in 10% of the isolated enterococci. When comparing species susceptibility, E. faecium isolates were significantly more resistant to penicillin, ampicillin, nitrofurantoin, and ciprofloxacin (p < 0.05). Eleven E. faecium isolates (92%) and 12 E. faecalis isolates (76%) were multidrug resistant.
本研究调查了克罗地亚西北部患有尿路感染的犬和猫的肠球菌的流行情况和药敏性。在这项研究中，实验室共收到 787 份尿液样本，其中 651 份来自犬，136 份来自猫。共有 260 份尿液样本（211 份来自犬，49 份来自猫）为细菌学阳性。其中 29 株分离株属于肠球菌属；22 株来自犬，7 株来自猫。通过 PCR 方法鉴定肠球菌分离株，其中 12 株为屎肠球菌，17 株为粪肠球菌。在犬中，鉴定出 16 株粪肠球菌和 6 株屎肠球菌，而在猫中，鉴定出 6 株屎肠球菌和 1 株粪肠球菌。通过 Kirby-Bauer 纸片扩散法测定了 9 种抗菌药物对肠球菌的药敏性：青霉素、氨苄西林、万古霉素、呋喃妥因、利福平、四环素、氯霉素、恩诺沙星、环丙沙星。还对这些分离株进行了高水平耐药性试验，包括链霉素和庆大霉素。肠球菌属的最高耐药性是对利福平（86%）和恩诺沙星（83%），其次是四环素和环丙沙星（69%）。对万古霉素的耐药率为 28%，最低的是氯霉素（17%）。76%的肠球菌分离株为多重耐药。17%的分离肠球菌对高水平链霉素耐药，10%的分离肠球菌对高水平庆大霉素耐药。在比较不同种属的药敏性时，屎肠球菌对青霉素、氨苄西林、呋喃妥因和环丙沙星的耐药性明显更高（p<0.05）。11 株屎肠球菌分离株（92%）和 12 株粪肠球菌分离株（76%）为多重耐药。
