谷氨酸脱氢酶人脑海马cDNA的分离。

Isolation of a human brain cDNA for glutamate dehydrogenase.

作者信息

Banner C, Silverman S, Thomas J W, Lampel K A, Vitkovic L, Huie D, Wenthold R J

出版信息

J Neurochem. 1987 Jul;49(1):246-52. doi: 10.1111/j.1471-4159.1987.tb03422.x.

DOI:10.1111/j.1471-4159.1987.tb03422.x

Abstract

A cDNA has been isolated from a human brain expression library using anti-bovine glutamate dehydrogenase (GDH) antibodies. The cDNA has an open reading frame of 774 nucleotides, which codes for 258 amino acids. The 258-amino-acid sequence is 95% homologous to the carboxy terminus of human liver GDH. This high degree of homology indicates that the cDNA codes for brain GDH. Fourteen differences between the amino acid sequence deduced from this cDNA and the sequence reported for human liver GDH suggest that there may be two active human GDH genes. A cRNA probe synthesized from the cDNA detects a 3.7-kilobase (kb) mRNA from human brain. Rat liver and kidney each contain two GDH mRNAs, 3.5 and 2.8 kb, respectively. The 3.5-kb transcript is prominent in rat brain, whereas the 2.8-kb transcript is barely detectable, a result suggesting that GDH gene expression is differentially controlled in rat brain.

摘要

利用抗牛谷氨酸脱氢酶（GDH）抗体从人脑表达文库中分离出了一个cDNA。该cDNA具有774个核苷酸的开放阅读框，编码258个氨基酸。这258个氨基酸的序列与人类肝脏GDH的羧基末端有95%的同源性。这种高度同源性表明该cDNA编码脑GDH。从这个cDNA推导的氨基酸序列与报道的人类肝脏GDH序列之间有14个差异，这表明可能存在两个有活性的人类GDH基因。由该cDNA合成的cRNA探针可检测到人脑3.7千碱基（kb）的mRNA。大鼠肝脏和肾脏分别含有两种GDH mRNA，分别为3.5 kb和2.8 kb。3.5 kb的转录本在大鼠脑中占主导地位，而2.8 kb的转录本几乎检测不到，这一结果表明GDH基因表达在大鼠脑中受到差异调控。