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基于比率型荧光探针的现场监测冷却肉腐败的硫化氢气体传感。

Hydrogen sulfide gas sensing toward on-site monitoring of chilled meat spoilage based on ratio-type fluorescent probe.

机构信息

School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Rd, 212013 Zhenjiang, Jiangsu, China.

School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Rd, 212013 Zhenjiang, Jiangsu, China.

出版信息

Food Chem. 2022 Dec 1;396:133654. doi: 10.1016/j.foodchem.2022.133654. Epub 2022 Jul 9.

DOI:10.1016/j.foodchem.2022.133654
PMID:35853371
Abstract

In this study, a fluorescence sensing platform for visual detection of hydrogen sulfide (HS) based on ratiometric fluorescent substances was developed to real-time monitor meat spoilage. The copper nanoclusters (CuNCs) and nitrogen-doped carbon quantum dots (CNQDs) were used as dual emission fluorescence materials. The fluorescence ratio of the two wavelengths decreased in the sulphide concentration range of 0-3 μmolL(exp), with a detection limit of 62.7 nmolL(exp). In order to capture hydrogen sulfide gas in the air, the ratio fluorescent material is loaded on the paper base. By processing the RGB value of the photo under UV light, the detection limit of the sensor is 4.35 ppt in the range of 0 ∼ 45.2 ppt HS gas concentration. This portable visual analysis greatly simplifies the steps of HS gas detection while ensuring sensor stability and sensitivity. It also provides a new method for HS detection during the meat storage process.

摘要

在这项研究中,开发了一种基于比率荧光物质的荧光传感平台,用于实时监测肉的腐败。该平台使用铜纳米簇(CuNCs)和氮掺杂碳量子点(CNQDs)作为双发射荧光材料。在硫化物浓度范围为 0-3 μmolL(exp)时,两个波长的荧光比率降低,检测限为 62.7 nmolL(exp)。为了捕获空气中的硫化氢气体,将比率荧光材料负载在纸基上。通过处理紫外光下的照片的 RGB 值,可以在 0 到 45.2 ppt HS 气体浓度范围内将传感器的检测限降低到 4.35 ppt。这种便携式可视分析方法大大简化了 HS 气体检测的步骤,同时保证了传感器的稳定性和灵敏度。它还为肉品贮藏过程中的 HS 检测提供了一种新方法。

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