Key Laboratory of Modern Agricultural Equipment and Technology, Ministry of Education, School of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, China; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Key Laboratory of Modern Agricultural Equipment and Technology, Ministry of Education, School of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, China.
Food Chem. 2022 Dec 1;396:133707. doi: 10.1016/j.foodchem.2022.133707. Epub 2022 Jul 16.
Zearalenone (ZEN) is a universal mycotoxin contaminant in corn and its products. A surface-enhanced Raman scattering (SERS) based test strip was proposed for the detection of ZEN, which had the advantages of simplicity, rapidity, and high sensitivity. Core-shell Au@AgNPs with embedded reporter molecules (4-MBA) were synthesized as SERS nanoprobe, which exhibited excellent SERS signals and high stability. The detection range of ZEN for corn samples was 10-1000 μg/kg with the limit of detection (LOD) of 3.6 μg/kg, which is far below the recommended tolerable level (60 μg/kg). More importantly, the SERS method was verified by HPLC in the application on corn samples contaminated with ZEN, and the coincidence rates were in the range of 86.06%-111.23%, suggesting a high accuracy of the SERS assay. Therefore, the SERS-based test strip with an analysis time of less than 15 min is a promising tool for accurate and rapid detection of ZEN-field contamination.
玉米及其制品中普遍存在的真菌毒素污染物——玉米赤霉烯酮（ZEN）。本研究提出了一种基于表面增强拉曼散射（SERS）的检测试纸条，用于 ZEN 的检测，其具有简便、快速和高灵敏度的优点。采用嵌入报告分子（4-MBA）的核壳型 Au@AgNPs 作为 SERS 纳米探针，具有优异的 SERS 信号和高稳定性。该方法对玉米样品中 ZEN 的检测范围为 10-1000μg/kg，检出限（LOD）为 3.6μg/kg，远低于建议的耐受水平（60μg/kg）。更重要的是，在对受 ZEN 污染的玉米样品的应用中，通过 HPLC 对 SERS 方法进行了验证，符合率在 86.06%-111.23%之间，表明 SERS 检测具有较高的准确性。因此，该 SERS 检测试纸条具有分析时间小于 15min 的特点，是一种用于准确快速检测田间污染 ZEN 的有前途的工具。
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