Yin Limei, Cai Jianrong, Ma Lixin, You Tianyan, Arslan Muhammad, Jayan Heera, Zou Xiaobo, Gong Yunyun
International Joint Research Laboratory of Intelligent Agriculture and Agri-products Processing, School of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, China; China Light Industry Key Laboratory of Food Intelligent Detection & Processing, School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
China Light Industry Key Laboratory of Food Intelligent Detection & Processing, School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Food Chem. 2024 Jul 15;446:138817. doi: 10.1016/j.foodchem.2024.138817. Epub 2024 Feb 20.
Aflatoxin B1 (AFB1) and zearalenone (ZEN) are two mycotoxins that often co-occur in corn. A surface-enhanced Raman scattering-based lateral flow immunoassay (SERS-LFIA) that can simultaneously detect AFB1 and ZEN in corn samples was developed employing the core-interlayer-satellite magnetic nanocomposites (FeO@PEI/Au@Ag) as dual-functional SERS tags. Under the optimal conditions, the detection ranges of AFB1 and ZEN in corn samples were 0.1-10 μg/kg and 4-400 μg/kg, respectively. Moreover, the test results for two mycotoxins in contaminated corn samples employing the suggested SERS-LFIA was in line with those of the HPLC technique. In view of its satisfactory sensitivity, accuracy, precision and short testing time (20 min), the developed system has a promising application prospect in the on-site simultaneous detection of AFB1 and ZEN.
黄曲霉毒素B1（AFB1）和玉米赤霉烯酮（ZEN）是两种经常共存于玉米中的霉菌毒素。利用核-夹层-卫星磁性纳米复合材料（FeO@PEI/Au@Ag）作为双功能表面增强拉曼散射（SERS）标签，开发了一种基于SERS的侧向流动免疫分析法（SERS-LFIA），可同时检测玉米样品中的AFB1和ZEN。在最佳条件下，玉米样品中AFB1和ZEN的检测范围分别为0.1-10μg/kg和4-400μg/kg。此外，采用所建议的SERS-LFIA对受污染玉米样品中两种霉菌毒素的检测结果与高效液相色谱（HPLC）技术的结果一致。鉴于其令人满意的灵敏度、准确性、精密度和较短的检测时间（20分钟），所开发的系统在现场同时检测AFB1和ZEN方面具有广阔的应用前景。
