基于磁性纳米复合材料的表面增强拉曼散射侧流条同时检测黄曲霉毒素B1和玉米赤霉烯酮的双重功能

Dual function of magnetic nanocomposites-based SERS lateral flow strip for simultaneous detection of aflatoxin B1 and zearalenone.

作者信息

Yin Limei, Cai Jianrong, Ma Lixin, You Tianyan, Arslan Muhammad, Jayan Heera, Zou Xiaobo, Gong Yunyun

机构信息

International Joint Research Laboratory of Intelligent Agriculture and Agri-products Processing, School of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, China; China Light Industry Key Laboratory of Food Intelligent Detection & Processing, School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.

China Light Industry Key Laboratory of Food Intelligent Detection & Processing, School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.

出版信息

Food Chem. 2024 Jul 15;446:138817. doi: 10.1016/j.foodchem.2024.138817. Epub 2024 Feb 20.

DOI:10.1016/j.foodchem.2024.138817

PMID:38401299

Abstract

Aflatoxin B1 (AFB1) and zearalenone (ZEN) are two mycotoxins that often co-occur in corn. A surface-enhanced Raman scattering-based lateral flow immunoassay (SERS-LFIA) that can simultaneously detect AFB1 and ZEN in corn samples was developed employing the core-interlayer-satellite magnetic nanocomposites (FeO@PEI/Au@Ag) as dual-functional SERS tags. Under the optimal conditions, the detection ranges of AFB1 and ZEN in corn samples were 0.1-10 μg/kg and 4-400 μg/kg, respectively. Moreover, the test results for two mycotoxins in contaminated corn samples employing the suggested SERS-LFIA was in line with those of the HPLC technique. In view of its satisfactory sensitivity, accuracy, precision and short testing time (20 min), the developed system has a promising application prospect in the on-site simultaneous detection of AFB1 and ZEN.

摘要

黄曲霉毒素B1（AFB1）和玉米赤霉烯酮（ZEN）是两种经常共存于玉米中的霉菌毒素。利用核-夹层-卫星磁性纳米复合材料（FeO@PEI/Au@Ag）作为双功能表面增强拉曼散射（SERS）标签，开发了一种基于SERS的侧向流动免疫分析法（SERS-LFIA），可同时检测玉米样品中的AFB1和ZEN。在最佳条件下，玉米样品中AFB1和ZEN的检测范围分别为0.1-10μg/kg和4-400μg/kg。此外，采用所建议的SERS-LFIA对受污染玉米样品中两种霉菌毒素的检测结果与高效液相色谱（HPLC）技术的结果一致。鉴于其令人满意的灵敏度、准确性、精密度和较短的检测时间（20分钟），所开发的系统在现场同时检测AFB1和ZEN方面具有广阔的应用前景。