温阳振衰颗粒对阿霉素诱导的 H9c2 心肌细胞损伤模型中线粒体自噬关键蛋白表达的影响。
Effects of Wenyang Zhenshuai Granules on the Expression of Key Mitochondrial Autophagy Proteins in the Doxorubicin-Induced Model of H9c2 Cardiomyocyte Injury.
机构信息
The First Affiliated Hospital of Hunan University of Chinese Medicine, Hunan, China.
Chengdu Xindu District Traditional Chinese Medicine Hospital, Sichuan, China.
出版信息
Bull Exp Biol Med. 2022 Jul;173(3):335-340. doi: 10.1007/s10517-022-05545-9. Epub 2022 Jul 20.
This study aimed to explore the effects of Wenyang Zhenshuai granules (WZG) on the morphology of cardiomyocytes, cell viability, and the expression of key mitochondrial autophagy proteins in the doxorubicin-induced model of H9c2 cardiomyocyte injury. Cardiomyocytes were cultured for 44 h and divided into 4 groups: intact control, doxorubicin-injured cells (DOX), doxorubicin-injured cells treated with WZG (DOX+WZG), and doxorubicin-injured cells treated with valsartan (DOX+valsartan; reference group). The morphology of cardiomyocytes was analyzed under an inverted microscope; cardiomyocyte survival rate was determined by MTT assay. The expression of the key mitochondrial autophagy proteins (PINK1, parkin, LC3-II, and prohibitin-2) was analyzed by Western blotting. WZG down-regulated the expression of the key mitochondrial autophagy proteins in DOX-injured cells, which may be one of the important mechanisms for regulating ventricular remodeling and cardiomyocyte apoptosis.
本研究旨在探讨温阳振衰颗粒（WZG）对阿霉素诱导的 H9c2 心肌细胞损伤模型中心肌细胞形态、细胞活力和关键线粒体自噬蛋白表达的影响。心肌细胞培养 44 h 后，分为 4 组：完整对照组、阿霉素损伤细胞组（DOX）、阿霉素损伤细胞加 WZG 处理组（DOX+WZG）和阿霉素损伤细胞加缬沙坦处理组（DOX+valsartan；参考组）。倒置显微镜下观察心肌细胞形态；MTT 法测定心肌细胞存活率。Western blot 法分析关键线粒体自噬蛋白（PINK1、parkin、LC3-II 和 prohibitin-2）的表达。WZG 下调 DOX 损伤细胞中关键线粒体自噬蛋白的表达，这可能是调节心室重构和心肌细胞凋亡的重要机制之一。
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