Go Jun, Park Hye-Yeon, Lee Da Woon, Maeng So-Young, Lee In-Bok, Seo Yun Jeong, An Jin-Pyo, Oh Won Keun, Lee Chul-Ho, Kim Kyoung-Shim
Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology, Gwahak-ro 125, Yuseong-gu, Daejeon, 34141, Republic of Korea.
Department of Biomaterials Science, College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, 50463, Republic of Korea.
Lab Anim Res. 2022 Jul 19;38(1):21. doi: 10.1186/s42826-022-00134-3.
Neuroinflammation plays an important role in cognitive decline and memory impairment in neurodegenerative disorders. Previously, we demonstrated that Humulus japonicus (HJ) has anti-inflammatory effects in rodent models of Alzheimer's disease and Parkinson's disease. The present study aimed to examine the protective potential of HJ extracts against lipopolysaccharide (LPS)-induced cognitive impairment and scopolamine-induced amnesia in mouse models. Cognitive improvement of mice was investigated by novel object recognition test. For analyzing effects on neuroinflammation, immunohistochemistry and quantitative real-time polymerase chain reaction (qRT-PCR) assays were performed.
We found that the oral administration of HJ significantly improved cognitive dysfunction induced by LPS in a novel object recognition test. The LPS-induced activation of microglia was notably decreased by HJ treatment in the cortex and hippocampus. HJ administration with LPS also significantly increased the mRNA expression of interleukin (IL)-10 and decreased the mRNA expression of IL-12 in the parietal cortex of mice. The increased expression of LPS-induced complement C1q B chain (C1bq) and triggering receptor expressed on myeloid cells 2 (Trem2) genes was significantly suppressed by HJ treatment. In addition, HJ administration significantly improved novel object recognition in a scopolamine-induced amnesia mouse model.
These findings revealed that HJ has a beneficial effect on cognitive impairment and neuroinflammation induced by systemic inflammation and on amnesia induced by scopolamine in mice.
神经炎症在神经退行性疾病的认知衰退和记忆障碍中起重要作用。此前，我们证明了葎草（HJ）在阿尔茨海默病和帕金森病的啮齿动物模型中具有抗炎作用。本研究旨在检测HJ提取物对小鼠模型中脂多糖（LPS）诱导的认知障碍和东莨菪碱诱导的失忆的保护潜力。通过新物体识别试验研究小鼠的认知改善情况。为分析对神经炎症的影响，进行了免疫组织化学和定量实时聚合酶链反应（qRT-PCR）检测。
我们发现，在新物体识别试验中，口服HJ可显著改善LPS诱导的认知功能障碍。HJ处理可显著降低皮质和海马中LPS诱导的小胶质细胞活化。HJ与LPS联合给药还可显著增加小鼠顶叶皮质中白细胞介素（IL）-10的mRNA表达，并降低IL-12的mRNA表达。HJ处理可显著抑制LPS诱导的补体C1q B链（C1bq）和髓样细胞表达的触发受体2（Trem2）基因表达的增加。此外，HJ给药可显著改善东莨菪碱诱导的失忆小鼠模型中的新物体识别。
这些发现表明，HJ对小鼠全身炎症诱导的认知障碍和神经炎症以及东莨菪碱诱导的失忆具有有益作用。
