Department of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, 009811, Iran.
Department of Microbiology, Tonekabon Branch, Islamic Azad University, Tonekabon, 009811, Iran.
J Biomed Nanotechnol. 2022 Apr 1;18(4):1196-1204. doi: 10.1166/jbn.2022.3331.
: To evaluate the application of Ag-Cu NPs as quorum sensing (QS) inhibitors and attenuate virulence expression to overcome the global crisis of multidrug-resistant (MDR) . : Ag-Cu NPs were synthesized by co-reduction of silver-nitrate and copper-nitrate (Ag:Cu = 1:1 0.75 M). In this cross-sectional study, a total of eighty clinical strains of were collected from patients with burns. The antibacterial and resistance pattern of the clinical isolated was determined using the microdilution and Kirby Bauer disk methods. The effect of sub-MIC of Ag-Cu NPs on the expression of lasI, exoS and toxA in five clinical isolates of imipenem-resistant was performed using qRT-PCR. : The characterization methods confirm the formation of the Ag-Cu alloy NPs with agglomerated spherical morphology and particle sizes of about 30-40 nm. We observed that the MIC and MBC of Ag-Cu alloy NPs against MDR was found to be 2.5 and 5 g ml, respectively. The effects of a sub-inhibitory concentration of Ag-Cu NPs on MDR QS and virulence-related genes showed that the expression level of QS regulatory and virulence genes significantly decreased in both MDR and reference strain under Ag-Cu sub-MIC treatment. : Ag-Cu NPs could be potentially used as a promising QS inhibitor and anti-virulence compound against .
为了评估 Ag-Cu NPs 作为群体感应（QS）抑制剂的应用，以减轻毒力表达，从而克服多药耐药（MDR）的全球危机。Ag-Cu NPs 通过硝酸银和硝酸铜（Ag:Cu = 1:1 0.75 M）的共还原合成。在这项横断面研究中，共从烧伤患者中收集了 80 株临床分离株。使用微量稀释和 Kirby Bauer 圆盘法测定临床分离株的抗菌作用和耐药模式。使用 qRT-PCR 检测亚 MIC 的 Ag-Cu NPs 对 5 株耐亚胺培南铜绿假单胞菌 lasI、exoS 和 toxA 表达的影响。表征方法证实了 Ag-Cu 合金 NPs 的形成，具有团聚的球形形态和约 30-40nm 的粒径。我们观察到 Ag-Cu 合金 NPs 对 MDR 铜绿假单胞菌的 MIC 和 MBC 分别为 2.5 和 5 g/ml。亚抑制浓度的 Ag-Cu NPs 对 MDR 铜绿假单胞菌 QS 和毒力相关基因的影响表明，在 Ag-Cu 亚 MIC 处理下，QS 调节和毒力基因的表达水平在 MDR 铜绿假单胞菌和参考菌株中均显著降低。Ag-Cu NPs 可能被用作一种有前途的 QS 抑制剂和抗毒力化合物，用于治疗铜绿假单胞菌。
