谷胱甘肽稳定的银纳米粒子对铜绿假单胞菌菌株中 las I 和 las R 基因表达的影响。
Effect of glutathione-stabilized silver nanoparticles on expression of las I and las R of the genes in Pseudomonas aeruginosa strains.
机构信息
Islamic Azad University,North Tehran Branch, Tehran, Iran.
Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
出版信息
Eur J Med Res. 2020 May 20;25(1):17. doi: 10.1186/s40001-020-00415-4.
BACKGROUND
Biofilm formation is regarded as a significant factor in the establishment of infections caused by Pseudomonas aeruginosa. P. aeruginosa is one of the most important causes of nosocomial infections. Today silver nanoparticles (Ag-NPs) are used as antimicrobials due to their well-known, chemical, biological, and physical properties. Exposure to nanoparticles could inhibit colonization of new bacteria onto the biofilm.
METHODS
In the present work, the green synthesis of Ag-NPs was performed using the alcoholic extract of Eucalyptus camaldulensis. Ag-NPs and glutathione-stabilized silver nanoparticles (GSH-Ag-NPs) were characterized using X-ray diffraction (XRD), dynamic light scattering (DLS), scanning electron microscope (SEM), and carbon, nitrogen, and hydrogen (CNH) and Fourier transform infrared spectroscopy (FTIR) techniques were applied to investigate the structure of the modified nanoparticles. Then, the antimicrobial and antibiofilm potential of the prepared Ag-NPs and GSH-Ag-NPs against P. aeruginosa strains was evaluated using microbroth dilution method and their effects on the expression of las I and las R genes.
RESULTS
In this study, a total of 50 P. aeruginosa isolates were recovered from clinical samples. According to the results, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) value of Ag-NPs against P. aeruginosa was determined to be 512-256 μg/ml, respectively, while the MIC and MBC value of GS-Ag-NPs against P. aeruginosa clinical strains was determined in a range of 128-256 μg/ml and 256-512 μg/ml, respectively. The mean expression level in las R, las I genes in P. aeruginosa strains treated with ½ MIC of Ag-NPs was decreased by -5.7 and -8fold, respectively. The mean expression levels of las R, las I genes in P. aeruginosa strains treated with ½ MIC of GS-Ag-NPs were decreased by -8.7 and -10fold, respectively (P < 0.05).
CONCLUSIONS
The results of our study showed that Ag-NPs and GS-Ag-NPs are highly effective against P. aeruginosa strains. Moreover, this study also proves the promising potential of using nanoparticles as anti-biofilm formation and antibacterial agents.
背景
生物膜的形成被认为是铜绿假单胞菌引起感染的重要因素。铜绿假单胞菌是医院获得性感染的最重要原因之一。如今，由于其众所周知的化学、生物和物理特性，银纳米粒子（Ag-NPs）被用作抗菌剂。纳米粒子的暴露可以抑制新细菌在生物膜上的定植。
方法
在本工作中，使用桉树叶的醇提物对 Ag-NPs 进行了绿色合成。使用 X 射线衍射（XRD）、动态光散射（DLS）、扫描电子显微镜（SEM）和碳、氮、氢（CNH）和傅里叶变换红外光谱（FTIR）技术对 Ag-NPs 和谷胱甘肽稳定的银纳米粒子（GSH-Ag-NPs）进行了表征，以研究修饰纳米粒子的结构。然后，采用微量肉汤稀释法评价了制备的 Ag-NPs 和 GSH-Ag-NPs 对铜绿假单胞菌菌株的抗菌和抗生物膜潜力，并检测了它们对 las I 和 las R 基因表达的影响。
结果
在这项研究中，从临床样本中总共回收了 50 株铜绿假单胞菌。结果表明，Ag-NPs 对铜绿假单胞菌的最小抑菌浓度（MIC）和最小杀菌浓度（MBC）值分别为 512-256μg/ml，而 GSH-Ag-NPs 对铜绿假单胞菌临床株的 MIC 和 MBC 值分别在 128-256μg/ml 和 256-512μg/ml 的范围内。Ag-NPs 处理后，las R、las I 基因在铜绿假单胞菌中的平均表达水平分别降低了-5.7 和-8 倍。GS-Ag-NPs 处理后，las R、las I 基因在铜绿假单胞菌中的平均表达水平分别降低了-8.7 和-10 倍（P<0.05）。
结论
我们的研究结果表明，Ag-NPs 和 GSH-Ag-NPs 对铜绿假单胞菌菌株具有高度的有效性。此外，这项研究还证明了纳米粒子作为抗生物膜形成和抗菌剂的有前途的潜力。
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