Zhang Yanmei, Wang Xiankuan, Li Yaxian, Liang Jianhe, Jiang Pinliang, Huang Qiaoling, Yang Yun, Duan Hongping, Dong Xiang, Rui Gang, Lin Changjian
State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Research Institute for Biomimetics and Soft Matter, Fujian Provincial Key Laboratory for Soft Functional Materials Research, College of Physical Science and Technology, Xiamen University, Xiamen 361005, China.
Regen Biomater. 2022 Jul 1;9:rbac046. doi: 10.1093/rb/rbac046. eCollection 2022.
Hierarchical surface structures with micro-nano scale play a crucial role in regulation of cell proliferation and osteogenic differentiation. It has been proven that cells are extremely sensitive to the nanoscaled structure and show multifarious phenotypes. Though a vital function of microstructure on osseointegration has been confirmed, the cell performances response to different microscaled structure is needed to be further dissected and in depth understood. In this work, the ordered micro-nano hierarchical structures with varying micro-scaled pits were precisely fabricated on titanium successfully by the combination of electrochemical, chemical etching and anodization as well. systematical assessments indicated that the micro-nano multilevel structures on titanium exhibited excellent cells adhesion and spreading ability, as well as steerable proliferation and osteogenic differentiation behaviors. It is shown that smaller micro-pits and lower roughness of the hierarchical structures enabled faster cell propagation. Despite cell growth was delayed on micro-nano titanium with relatively larger cell-match-size micro-pits and roughness, osteogenic-specific genes were significantly elevated. Furthermore, the alkaline phosphatase activity, collagen secretion and extracellular matrix mineralization of MC3T3-E1 on multi-scaled titanium were suppressed by a large margin after adding IWP-2 (an inhibitor of Wnt/β-catenin signal pathway), indicating this pathway played a crucial part in cell osteogenic differentiation modulated by micro-nano structures.
具有微纳尺度的分级表面结构在细胞增殖和骨生成分化的调控中起着至关重要的作用。已经证明，细胞对纳米尺度的结构极其敏感，并表现出多种表型。尽管微观结构在骨整合中的重要作用已得到证实，但细胞对不同微观尺度结构的反应仍需进一步剖析和深入了解。在这项工作中，通过电化学、化学蚀刻和阳极氧化相结合的方法，成功地在钛表面精确制备了具有不同微尺度凹坑的有序微纳分级结构。系统评估表明，钛表面的微纳多级结构表现出优异的细胞粘附和铺展能力，以及可控的增殖和骨生成分化行为。结果表明，分级结构中较小的微坑和较低的粗糙度能使细胞更快地增殖。尽管在具有相对较大细胞匹配尺寸微坑和粗糙度的微纳钛表面细胞生长延迟，但成骨特异性基因显著上调。此外，添加IWP-2（一种Wnt/β-连环蛋白信号通路抑制剂）后，多尺度钛表面MC3T3-E1细胞的碱性磷酸酶活性、胶原蛋白分泌和细胞外基质矿化受到大幅抑制，表明该信号通路在微纳结构调节的细胞成骨分化中起关键作用。
Zotero 插件
