Construction of Complex Structures Containing Micro-Pits and Nano-Pits on the Surface of Titanium for Cytocompatibility Improvement.

The surface topography of medical implants plays an important role in the regulation of cellular responses. Microstructure and nanostructure surfaces have been proved to enhance cell spreading and proliferation with respect to smooth surfaces. In this study, we fabricated a new structure including micro-pits and nano-pits on the surface of titanium via sandblasting, acid etching and chemical oxidation to investigate the influence of composite structures on cell behavior. Meanwhile, the surface properties and corrosion resistance of treated samples were also tested. The micro/nanostructured titanium surface comprising of micro-pits and nano-pits presented enhanced roughness and hydrophilicity. In addition, the corrosion resistance of the titanium substrate with micro-pits and nano-pits was significantly improved compared to that of polished titanium. More importantly, the micro/nanostructured titanium surface proved a good interfacial environment to promote osteoblast functions such as cell adhesion and spreading. Taken together, these results showed that the construction of micro/nanostructure on the titanium surface is an effective modification strategy to improve osteoblast cell responses.