Unit of Forensic Medicine, Department of Diagnostics and Public Health, University of Verona, Verona, Italy.
World-Class Research Center "Digital biodesign and personalized healthcare", Sechenov First Moscow State Medical University, Moscow, Russia.
Drug Test Anal. 2022 Oct;14(10):1773-1778. doi: 10.1002/dta.3346. Epub 2022 Jul 21.
Adherence to therapy is the key to a successful therapeutic intervention, especially in cardiovascular diseases in which a lack of adherence may have serious consequences in terms morbidity and/or mortality. In this context, hair analysis can be an excellent tool to monitor adherence to therapy. Indeed, drugs present in blood are incorporated into the hair matrix, where drugs and metabolites can stay unaltered for a long time protected from metabolism and degradation. In the present study, a simple, specific, and sensitive ultra-high performance liquid-chromatography-tandem mass spectrometry (UHPLC-MS/MS) method set up to determine in human hair seven beta-blockers (viz., metoprolol, sotalol, labetalol, atenolol, nebivolol, bisoprolol, and nadolol) and two calcium-channel blockers (lercanidipine and amlodipine), which are widely prescribed to treat medium-to-severe hypertensive conditions. The optimized method was successfully validated in terms of accuracy, repeatability, reproducibility, matrix effect and extraction recovery. Moreover, the applicability of the method was evaluated by analyzing 34 real samples of hair obtained from patients under long-term therapy with calcium channel blockers and beta-blockers.
治疗的依从性是成功治疗干预的关键，尤其是在心血管疾病中，不依从治疗可能会导致严重的发病率和/或死亡率后果。在这种情况下，毛发分析可以成为监测治疗依从性的一个极好工具。事实上，血液中的药物被整合到毛发基质中，药物和代谢物在那里可以长时间保持不变，免受代谢和降解的影响。在本研究中，建立了一种简单、特异和灵敏的超高效液相色谱-串联质谱（UHPLC-MS/MS）方法，用于测定人发中的七种β受体阻滞剂（即美托洛尔、索他洛尔、拉贝洛尔、阿替洛尔、比索洛尔、贝那普利和纳多洛尔）和两种钙通道阻滞剂（乐卡地平氨氯地平），这些药物广泛用于治疗中重度高血压。该优化方法在准确性、重复性、重现性、基质效应和提取回收率方面均得到了验证。此外，还通过分析 34 例来自长期接受钙通道阻滞剂和β受体阻滞剂治疗的患者的实际毛发样本，评估了该方法的适用性。
