Department of Life Science and Applied Chemistry, Nagoya Institute of Technology, Showa-ku, Nagoya, Japan.
Department of Chemistry, Graduate School of Science, Osaka University, Osaka, Japan.
Methods Mol Biol. 2022;2501:207-228. doi: 10.1007/978-1-0716-2329-9_10.
Vibrational spectroscopy such as FTIR and Raman spectroscopy is a powerful, sensitive, and informative method for studying protein structural changes in rhodopsins during their functions. The usefulness has been historically proven for the study of bacteriorhodopsin and bovine rhodopsin before their structural determination of rhodopsins. We now have atomic structures of many animal and microbial rhodopsins, and it is now important to know the structural dynamics of rhodopsins for function. FTIR and Raman spectroscopy provides useful information for this aim. In this chapter, we introduce the methods of FTIR and resonance Raman spectroscopy applied to rhodopsins. These vibrational methods offer deeper understanding on the mechanism how rhodopsins change their structures for function.
振动光谱学，如傅里叶变换红外（FTIR）和拉曼光谱学，是研究视紫红质在功能过程中蛋白质结构变化的一种强大、敏感和信息丰富的方法。在视紫红质结构确定之前，该方法已经在细菌视紫红质和牛视紫红质的研究中得到了历史验证。现在，我们已经拥有了许多动物和微生物视紫红质的原子结构，因此了解视紫红质的结构动力学对于其功能非常重要。FTIR 和共振拉曼光谱学为实现这一目标提供了有用的信息。在本章中，我们介绍了应用于视紫红质的 FTIR 和共振拉曼光谱学方法。这些振动方法对视紫红质如何改变其结构以发挥功能的机制提供了更深入的了解。
