Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, University of Jinan, Jinan, Shandong 250022, China.
State Key Laboratory of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing 210023, China.
Anal Chem. 2022 Aug 2;94(30):10651-10658. doi: 10.1021/acs.analchem.2c00889. Epub 2022 Jul 20.
An innovative self-powered microfluidic photoelectrochemical (PEC) aptasensor was developed that uses photoactive AgBr/CuBiO (ACO) composites as the photocathode matrix for ultrasensitive detection of ciprofloxacin (CIP) and ofloxacin (OFL). The formation of direct Z-scheme heterojunctions in ACO composites greatly aided electron/hole pair separation. Meanwhile, ZnInS-decorated CdS nanorod arrays (CZIS) as the photoanode were used instead of a platinum counter electrode to provide electrons. The "signal-off" CIP detection was accomplished through the steric hindrance effect in the photoanode due to the combination of aptamer and CIP. To increase the cathodic photocurrent intensity for OFL determination, controlled release of luminol was first used. Luminol molecules were successfully embedded in the porous structure of silicon dioxide nanospheres (PSiO) by the electrostatic adsorption between PSiO and aptamer. The luminol released by specific recognition between OFL and aptamer could not only react with •O but also produce chemiluminescence emission, resulting in the "signal-on" state. Because of the signal "on-off-on", the proposed aptasensor exhibited wide linear ranges for CIP (0.001-100 ng/mL) and OFL (0.0005-100 ng/mL) detection. Furthermore, the low detection limits of CIP (0.06 pg/mL) and OFL (0.022 pg/mL) could achieve the ultrasensitive analysis.
一种创新的自供电微流控光电化学（PEC）适体传感器被开发出来，该传感器使用光活性 AgBr/CuBiO（ACO）复合材料作为光电阴极基质，用于超灵敏检测环丙沙星（CIP）和氧氟沙星（OFL）。在 ACO 复合材料中形成直接 Z 型异质结极大地促进了电子/空穴对的分离。同时，ZnInS 修饰的 CdS 纳米棒阵列（CZIS）作为光阳极被用于代替铂对电极来提供电子。通过适配体与 CIP 的结合在光阳极中产生空间位阻效应，实现了“信号关闭”的 CIP 检测。为了提高 OFL 测定的阴极光电流强度，首先使用了鲁米诺的控制释放。通过 PSiO 和适配体之间的静电吸附，成功地将鲁米诺分子嵌入到二氧化硅纳米球（PSiO）的多孔结构中。由于适配体与 OFL 之间的特异性识别而释放的鲁米诺不仅可以与•O 反应，还可以产生化学发光发射，从而呈现“信号开启”状态。由于信号“开-关-开”，所提出的适体传感器表现出对 CIP（0.001-100ng/mL）和 OFL（0.0005-100ng/mL）检测的宽线性范围。此外，CIP（0.06pg/mL）和 OFL（0.022pg/mL）的低检测限可实现超灵敏分析。
