Hoengenaert Lennart, Wouters Marlies, Kim Hoon, De Meester Barbara, Morreel Kris, Vandersyppe Steven, Pollier Jacob, Desmet Sandrien, Goeminne Geert, Ralph John, Boerjan Wout, Vanholme Ruben
Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.
Center for Plant Systems Biology, VIB, Ghent, Belgium.
Sci Adv. 2022 Jul 15;8(28):eabo5738. doi: 10.1126/sciadv.abo5738. Epub 2022 Jul 13.
Lignin is the main factor limiting the enzymatic conversion of lignocellulosic biomass into fermentable sugars. To reduce the recalcitrance engendered by the lignin polymer, the coumarin scopoletin was incorporated into the lignin polymer through the simultaneous expression of () and () in lignifying cells in . The transgenic lines overproduced scopoletin and incorporated it into the lignin polymer, without adversely affecting plant growth. About 3.3% of the lignin units in the transgenic lines were derived from scopoletin, thereby exceeding the levels of the traditional hydroxyphenyl units. Saccharification efficiency of alkali-pretreated scopoletin-overproducing lines was 40% higher than for wild type.
木质素是限制木质纤维素生物质酶促转化为可发酵糖的主要因素。为降低木质素聚合物产生的顽固性，通过在[具体植物]木质化细胞中同时表达[具体基因1]和[具体基因2]，将香豆素东莨菪素掺入木质素聚合物中。转基因品系过量产生东莨菪素并将其掺入木质素聚合物中，而不会对植物生长产生不利影响。转基因品系中约3.3%的木质素单元源自东莨菪素，从而超过了传统羟苯基单元的水平。碱预处理的过量产生东莨菪素品系的糖化效率比野生型高40%。
