Clemmesen L, Jørgensen O S, Hemmingsen R, Barry D I, Bolwig T G
J Psychiatr Res. 1987;21(2):171-83. doi: 10.1016/0022-3956(87)90017-3.
The brain marker proteins, D1, D2, and D3, localised to neuronal membranes, and mitochondrial and cytoplasmic marker proteins (MM and CM), were studied during 1-6 days (short term) intragastrically-induced severe ethanol intoxication and during 1 month (long-term) ethanol intoxication established by a liquid diet regimen. The concentrations of the same brain proteins were also measured during withdrawal from the ethanol intoxication periods. Three categories of effect were encountered: decreased concentration of brain marker proteins during severe short-term intoxication the effect being most marked for D3, possibly indicating degradation of mature synapses; increased concentration of proteins D2 and MM during withdrawal, the D2 changes possibly indicating formation of new synapses; increased concentration of D1 protein and MM during long-term intoxication. We suggest that the changes in brain marker proteins reflect dynamic changes of subcellular neuronal structures which may form a part of the basis of functional tolerance to and physical dependence upon ethanol or the reversion of these states after withdrawal of ethanol.
研究了定位于神经元膜的脑标记蛋白D1、D2和D3，以及线粒体和细胞质标记蛋白（MM和CM），研究时间为通过灌胃诱导的1 - 6天（短期）严重乙醇中毒期间，以及通过液体饮食方案建立的1个月（长期）乙醇中毒期间。在乙醇中毒期戒断期间也测量了相同脑蛋白的浓度。发现了三类影响：严重短期中毒期间脑标记蛋白浓度降低，其中D3的影响最为明显，这可能表明成熟突触的降解；戒断期间蛋白D2和MM浓度增加，D2的变化可能表明新突触的形成；长期中毒期间D1蛋白和MM浓度增加。我们认为，脑标记蛋白的变化反映了亚细胞神经元结构的动态变化，这可能构成对乙醇功能耐受性和身体依赖性的部分基础，或者是乙醇戒断后这些状态逆转的部分基础。
