Heyner S, Komar J G
J Reprod Immunol. 1987 Jan;10(1):43-54. doi: 10.1016/0165-0378(87)90049-0.
Mice of the H-2b haplotype were mated with males of the same MHC haplotype, but differing at multiple minor histocompatibility loci. Mice were bled during each pregnancy and at 2-day intervals post-partum. The sera were assayed by indirect immunofluorescence for evidence of a humoral immune response to paternal minor histocompatibility antigens. Alloantibody was first detected in the post-partum period following the third pregnancy, and was also detected during the fourth pregnancy. Thereafter, alloantibody levels dropped and by the post-partum period following the fifth pregnancy, fell to control values. Assays on a panel of cells from mice of different inbred strains revealed specificity of the alloantibody to H-3.1, H-4.1 and H-7.1 antigens. A conventional dye exclusion cytotoxicity test revealed the pregnancy-induced alloantibody did not exhibit complement-dependent cytotoxicity. These findings are discussed in relation to the regulation and functional significance of the humoral immune response in allogeneic pregnancy.
将具有H-2b单倍型的小鼠与具有相同MHC单倍型但在多个次要组织相容性位点存在差异的雄性小鼠进行交配。在每次怀孕期间以及产后每隔2天采集小鼠血液。通过间接免疫荧光法检测血清，以寻找针对父本次要组织相容性抗原的体液免疫反应证据。同种抗体首先在第三次怀孕后的产后期间被检测到，在第四次怀孕期间也被检测到。此后，同种抗体水平下降，到第五次怀孕后的产后期间，降至对照值。对来自不同近交系小鼠的一组细胞进行检测，揭示了同种抗体对H-3.1、H-4.1和H-7.1抗原的特异性。传统的染料排除细胞毒性试验表明，怀孕诱导的同种抗体不表现出补体依赖性细胞毒性。结合同种异体妊娠中体液免疫反应的调节和功能意义对这些发现进行了讨论。
