Department of Biotechnology and Genetic Engineering, Jordan University of Science and Technology, Irbid, Jordan.
Department of Nutrition and Food Technology, Jordan University of Science and Technology, Irbid, Jordan.
J Appl Microbiol. 2022 Oct;133(4):2528-2546. doi: 10.1111/jam.15723. Epub 2022 Aug 5.
The aim was to characterize a collection of Cronobacter sakazakii isolates collected from various origins in Jordan.
The isolates were characterized using 16S rRNA sequencing, DNA microarray, multi-locus sequence typing (MLST), O-serotyping, virulence gene identification and antibiotic susceptibility testing. The identities and phylogenetic relatedness revealed that C. sakazakii sequence type 4 (ST4) and Csak O:1 serotype were the most prevalent STs and serovars amongst these C. sakazakii strains. PCR screening of putative virulence genes showed that the siderophore-interacting protein gene (sip) and iron acquisition gene clusters (eitCBAD and iucABCD/iutA) were the most detected genes with noticeable variability in the type 6 secretion system (T6SS) and filamentous hemagglutinin/adhesion (FHA) gene loci. The antibiotic resistance profiles revealed that the majority of the isolates were susceptible to all antibiotics used despite harbouring a class C β-lactamase resistance gene.
The results described in this report provide additional insights about the considerable genotypic and phenotypic heterogeneity within C. sakazakii.
The information reported in this study might be of great value in understanding the origins of C. sakazakii isolates, in addition to their diversity and variability, which might be helpful in preventing future outbreaks of this pathogen.
本研究旨在对从约旦各地收集的一批阪崎克罗诺杆菌分离株进行特征描述。
采用 16S rRNA 测序、DNA 微阵列、多位点序列分型（MLST）、O 血清型分型、毒力基因鉴定和抗生素药敏试验对分离株进行特征描述。鉴定和系统发育分析结果表明，C. sakazakii 序列型 4（ST4）和 Csak O:1 血清型是这些 C. sakazakii 菌株中最常见的 ST 和血清型。对推定毒力基因的 PCR 筛选显示，铁载体相互作用蛋白基因（sip）和铁获取基因簇（eitCBAD 和 iucABCD/iutA）是最常检测到的基因，而 6 型分泌系统（T6SS）和丝状血凝素/黏附素（FHA）基因座的基因存在明显的变异性。抗生素耐药谱显示，尽管大多数分离株携带有类 Cβ-内酰胺酶耐药基因，但对所有使用的抗生素均敏感。
本报告中描述的结果提供了有关 C. sakazakii 内部相当大的基因型和表型异质性的更多见解。
本研究报告的信息对于了解 C. sakazakii 分离株的来源、多样性和变异性具有重要价值，这可能有助于预防该病原体的未来爆发。
