Ismail G, Noor Embi M, Omar O, Allen J C, Smith C J
J Med Microbiol. 1987 Jun;23(4):353-7. doi: 10.1099/00222615-23-4-353.
The development of monoclonal antibody and enzyme-linked immunosorbent assay (ELISA) techniques has made possible the detection of specific antigens at extremely low concentrations. Diagnosis of recalcitrant diseases such as melioidosis depends upon either early isolation and identification of the causative organism or the identification of a specific marker antigen, Pseudomonas pseudomallei exotoxin, in serum; the latter is better because it allows more rapid and simple diagnosis. A method of detecting exotoxin concentrations of greater than 16 ng/ml by an ELISA based on a monoclonal antitoxin is here described; it is significantly more sensitive than the mouse lethality test (lower threshold 30 micrograms/ml) currently in use and an in-vitro cytotoxicity test (lower threshold 10 micrograms/ml) that we have developed and describe here.
单克隆抗体和酶联免疫吸附测定（ELISA）技术的发展使得检测极低浓度的特定抗原成为可能。对类鼻疽等顽固性疾病的诊断，要么依赖于早期分离和鉴定致病生物体，要么依赖于血清中特定标志物抗原——类鼻疽假单胞菌外毒素的鉴定；后者更好，因为它能实现更快速、简单的诊断。本文描述了一种基于单克隆抗毒素的ELISA检测外毒素浓度大于16 ng/ml的方法；该方法比目前使用的小鼠致死试验（下限30微克/毫升）以及我们在此开发并描述的体外细胞毒性试验（下限10微克/毫升）显著更灵敏。
