Taratima Worasitikulya, Chomarsa Titirat, Maneerattanarungroj Pitakpong
Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.
Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Scientifica (Cairo). 2022 Jul 11;2022:5616683. doi: 10.1155/2022/5616683. eCollection 2022.
Soil salinity limits plant growth and production. This research investigated a suitable medium for callus induction and plantlet regeneration in the Luem Pua rice cultivar. The effect of salt stress on seedling growth was determined using culture and soil conditions. An efficient protocol for callus induction has been developed by culture sterilized seeds on the Murashige and Skoog (MS, 1962) medium containing 0.5 mg/l benzyladenine (BA) with 1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) that resulted in a 100% callus induction. Plantlet regeneration percentage of 49% was recorded on the MS medium containing 4 mg/l BA with 0.5 mg/l 1-naphthaleneacetic acid (NAA) after 4 weeks. For salt stress investigation, the calli were treated on an induction medium containing various concentrations of NaCl (0, 50, 100, 150, and 200 mM), while two-week-old rice seedlings were planted in soil and treated with the same concentration of NaCl for 4 weeks. culture revealed that callus survival percentage decreased when NaCl concentration increased, similar to soil culture. Seedling growth under salinity treatment also decreased when NaCl concentration increased, while other physiological parameters such as total chlorophyll, chlorophyll a, chlorophyll b, green intensity, and chlorophyll fluorescence under light conditions increased under salinity stress. These changes define the growth and physiological salinity tolerance characteristics of Luem Pua rice calli and seedlings. They can be utilized as a baseline for demand-driven rice propagation, providing useful information that can be combined with other agronomic features in rice development or breeding programs to improve the flexibility of abiotic stress-tolerant cultivars.
土壤盐度限制了植物的生长和产量。本研究调查了一种适合用于陆恩普阿水稻品种愈伤组织诱导和植株再生的培养基。利用培养和土壤条件测定了盐胁迫对幼苗生长的影响。通过在含有0.5毫克/升苄基腺嘌呤（BA）和1毫克/升2,4-二氯苯氧乙酸（2,4-D）的Murashige和Skoog（MS，1962）培养基上培养无菌种子，开发出了一种高效的愈伤组织诱导方案，该方案的愈伤组织诱导率达到了100%。4周后，在含有4毫克/升BA和0.5毫克/升1-萘乙酸（NAA）的MS培养基上，植株再生率记录为49%。为了进行盐胁迫研究，将愈伤组织在含有不同浓度NaCl（0、50、100、150和200毫摩尔）的诱导培养基上进行处理，同时将两周大的水稻幼苗种植在土壤中，并用相同浓度的NaCl处理4周。培养结果显示，与土壤培养情况类似，当NaCl浓度增加时，愈伤组织的存活率下降。盐胁迫处理下，随着NaCl浓度的增加，幼苗生长也下降，而在盐胁迫下，其他生理参数如总叶绿素、叶绿素a、叶绿素b、绿色强度和光照条件下的叶绿素荧光增加。这些变化定义了陆恩普阿水稻愈伤组织和幼苗的生长及生理盐耐受性特征。它们可作为需求驱动型水稻繁殖的基线，提供有用信息，这些信息可与水稻发育或育种计划中的其他农艺特征相结合，以提高非生物胁迫耐受性品种的灵活性。
